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Osteoinductive activity of insulin-functionalized cell culture surfaces obtained using diazonium chemistry

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Osteoinductive activity of insulin-functionalized cell culture surfaces obtained using diazonium chemistry

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dc.contributor.author Mikulska, Anna pl
dc.contributor.author Filipowska, Joanna pl
dc.contributor.author Osyczka, Anna [SAP11019024] pl
dc.contributor.author Nowakowska, Maria [SAP11005597] pl
dc.contributor.author Szczubiałka, Krzysztof [SAP11014318] pl
dc.date.accessioned 2015-03-31T10:43:22Z
dc.date.available 2015-03-31T10:43:22Z
dc.date.issued 2015 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/4410
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Osteoinductive activity of insulin-functionalized cell culture surfaces obtained using diazonium chemistry pl
dc.type JournalArticle pl
dc.identifier.weblink https://www.frontiersin.org/articles/10.3389/fchem.2014.00117/full pl
dc.abstract.en Polymeric surfaces suitable for cell culture (DR/Pec) were constructed from diazoresin (DR) and pectin (Pec) in a form of ultrathin films using the layer-by-layer (LbL) technique. The surfaces were functionalized with insulin using diazonium chemistry. Such functionalized surfaces were used to culture human mesenchymal stem cells (hMSCs) to assess their suitability for bone tissue engineering and regeneration. The activity of insulin immobilized on the surfaces (DR/Pec/Ins) was compared to that of insulin dissolved in the culture medium. Human MSC grown on insulin-immobilized DR/Pec surfaces displayed increased proliferation and higher osteogenic activity. The latter was determined by means of alkaline phosphatase (ALP) activity, which increases at early stages of osteoblasts differentiation. Insulin dissolved in the culture medium did not stimulate cell proliferation and its osteogenic activity was significantly lower. Addition of recombinant human bone morphogenetic protein 2 (rhBMP-2) to the culture medium further increased ALP activity in hMSCs indicating additive osteogenic action of immobilized insulin and rhBMP-2. pl
dc.description.volume 2 pl
dc.description.publication 0,8 pl
dc.identifier.doi 10.3389/fchem.2014.00117 pl
dc.identifier.eissn 2296-2646 pl
dc.title.journal Frontiers in Chemistry pl
dc.language.container eng pl
dc.date.accession 2019-04-24 pl
dc.affiliation Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Zoologii pl
dc.subtype Article pl
dc.identifier.articleid 117 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl


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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa