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Human fibrinogen adsorption on positively charged latex particles

Human fibrinogen adsorption on positively charged ...

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dc.contributor.author Żeliszewska, Paulina pl
dc.contributor.author Bratek-Skicki, Anna pl
dc.contributor.author Adamczyk, Zbigniew pl
dc.contributor.author Cieśla, Michał [SAP11018214] pl
dc.date.accessioned 2015-03-11T08:01:43Z
dc.date.available 2015-03-11T08:01:43Z
dc.date.issued 2014 pl
dc.identifier.issn 0743-7463 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/3682
dc.language eng pl
dc.rights Dodaję tylko opis bibliograficzny *
dc.rights.uri *
dc.title Human fibrinogen adsorption on positively charged latex particles pl
dc.type JournalArticle pl
dc.description.physical 11165-11174 pl
dc.abstract.other Fibrinogen (Fb) adsorption on positively charged latex particles (average diameter of 800 nm) was studied using the microelectrophoretic and the concentration depletion methods based on AFM imaging. Monolayers on latex were adsorbed from diluted bulk solutions at pH 7.4 and an ionic strength in the range of 10^{−3} to 0.15 M where fibrinogen molecules exhibited an average negative charge. The electrophoretic mobility of the latex after controlled fibrinogen adsorption was systematically measured. A monotonic decrease in the electrophoretic mobility of fibrinogen-covered latex was observed for all ionic strengths. The results of these experiments were interpreted according to the three-dimensional electrokinetic model. It was also determined using the concentration depletion method that fibrinogen adsorption was irreversible and the maximum coverage was equal to 0.6 mg m^{−2} for ionic strength 10^{−3} M and 1.3 mg m^{−2} for ionic strength 0.15 M. The increase of the maximum coverage was confirmed by theoretical modeling based on the random sequential adsorption approach. Paradoxically, the maximum coverage of fibrinogen on positively charged latex particles was more than two times lower than the maximum coverage obtained for negative latex particles (3.2 mg m^{−2}) at pH 7.4 and ionic strength of 0.15 M. This was interpreted as a result of the side-on adsorption of fibrinogen molecules with their negatively charged core attached to the positively charged latex surface. The stability and acid base properties of fibrinogen monolayers on latex were also determined in pH cycling experiments where it was observed that there were no irreversible conformational changes in the fibrinogen monolayers. Additionally, the zeta potential of monolayers was more positive than the zeta potential of fibrinogen in the bulk, which proves a heterogeneous charge distribution. These experimental data reveal a new, side-on adsorption mechanism of fibrinogen on positively charged surfaces and confirmed the decisive role of electrostatic interactions in this process. pl
dc.subject.en adsorption pl
dc.subject.en fibrinogen pl
dc.subject.en RSA pl
dc.description.volume 30 (37) pl
dc.identifier.doi 10.1021/la5025668 pl
dc.identifier.eissn 1520-5827 pl
dc.title.journal Langmuir pl
dc.language.container eng pl
dc.affiliation Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego pl
dc.subtype Article pl
dc.rights.original bez licencji pl
.pointsMNiSW [2014 A]: 35

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