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Carbon monoxide shifts energetic metabolism from glycolysis to oxidative phosphorylation in endothelial cells

Carbon monoxide shifts energetic metabolism from ...

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dc.contributor.author Kaczara, Patrycja [SAP14002693] pl
dc.contributor.author Motterlini, Roberto pl
dc.contributor.author Kuś, Kamil [SAP14006230] pl
dc.contributor.author Zakrzewska, Agnieszka [SAP14003150] pl
dc.contributor.author Abramov, Andrey Y. pl
dc.contributor.author Chłopicki, Stefan [SAP14004240] pl
dc.date.accessioned 2016-12-19T08:31:14Z
dc.date.available 2016-12-19T08:31:14Z
dc.date.issued 2016 pl
dc.identifier.issn 0014-5793 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/34015
dc.language eng pl
dc.title Carbon monoxide shifts energetic metabolism from glycolysis to oxidative phosphorylation in endothelial cells pl
dc.type JournalArticle pl
dc.description.physical 3469-3480 pl
dc.abstract.en Carbon monoxide (CO) modulates mitochondrial respiration, but the mechanisms involved are not completely understood. The aim of the present study was to investigate the acute effects of CO on bioenergetics and metabolism in intact EA.hy926 endothelial cells using live cell imaging techniques. Our findings indicate that CORM-401, a compound that liberates CO, reduces ATP production from glycolysis, and induces a mild mitochondrial depolarization. In addition, CO from CORM-401 increases mitochondrial calcium and activates complexes I and II. The subsequent increase in mitochondrial respiration leads to ATP production through oxidative phosphorylation. Thus, our results show that nonactivated endothelial cells rely primarily on glycolysis, but in the presence of CO, mitochondrial Ca2+ increases and activates respiration that shifts the metabolism of endothelial cells from glycolysis- to oxidative phosphorylation-dependent ATP production. pl
dc.subject.en carbon monoxide pl
dc.subject.en CO-releasing molecule pl
dc.subject.en endothelium pl
dc.subject.en glycolysis pl
dc.subject.en oxidative phosphorylation pl
dc.subject.en respiration pl
dc.description.volume 590 pl
dc.description.number 20 pl
dc.description.points 30 pl
dc.identifier.doi 10.1002/1873-3468.12434 pl
dc.identifier.eissn 1873-3468 pl
dc.title.journal FEBS Letters pl
dc.language.container eng pl
dc.affiliation Pion Prorektora ds. badań naukowych i funduszy strukturalnych : Jagiellońskie Centrum Rozwoju Leków pl
dc.subtype Article pl
dc.rights.original bez licencji pl
.pointsMNiSW [2016 A]: 30

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