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Towards the understanding of the behavior of single-chained ether phospholipids in model biomembranes : interactions with phosphatidylethanolamines in Langmuir monolayers

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Towards the understanding of the behavior of single-chained ether phospholipids in model biomembranes : interactions with phosphatidylethanolamines in Langmuir monolayers

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dc.contributor.author Hąc-Wydro, Katarzyna [SAP11018762] pl
dc.contributor.author Flasiński, Michał [SAP13036957] pl
dc.contributor.author Wydro, Paweł [SAP11018949] pl
dc.contributor.author Dynarowicz-Łątka, Patrycja [SAP11012997] pl
dc.date.accessioned 2015-02-26T09:10:05Z
dc.date.available 2015-02-26T09:10:05Z
dc.date.issued 2012 pl
dc.identifier.issn 0927-7765 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/3343
dc.language eng pl
dc.title Towards the understanding of the behavior of single-chained ether phospholipids in model biomembranes : interactions with phosphatidylethanolamines in Langmuir monolayers pl
dc.type JournalArticle pl
dc.description.physical 162-170 pl
dc.abstract.en Three single-chained ether lipids of comparable chemical structure but different biological activities (namely natural platelet activating factor – PAF, its deacetylated precursor – lyso-PAF and synthetic compound – edelfosine – ED) have been investigated in mixed Langmuir monolayers with phos- phatidylethanolamines, PEs (DSPE, SOPE and DOPE), serving as model of inner shell of cellular membrane. The aim of undertaken studies was to verify the correlation between minor differences in chemical struc- tures of the investigated ether lipids and their behavior in membrane-mimicking environment. To reach this goal the interactions between particular ether lipids and PEs have been analyzed with G Exc values derived from the surface pressure–area isotherms. To get insight into miscibility between film com- ponents, Brewster angle microscopy, enabling direct visualization of monolayers structure, has been applied. The obtained results prove significant differences in both mixing properties and the interactions in the investigated systems. On one hand, they are related to the structure of polar head-groups of the studied ether lipids, which determine their capability of hydrogen bond(s) formation with head-groups of PEs. Edelfosine, lacking this property, interacts with PEs the most unfavorably among all the inves- tigated compounds. Another important parameter in this context is the structure of PEs monolayers – the most closely packed DSPE film was found to be most unfavorable for incorporation of ether lipid molecules. Our results prove that the analysis of the interaction between ether lipids and components of biomembrane in Langmuir monolayers is a potent method to explain differences in biological activity of the investigated ether lipids. pl
dc.subject.en single-chained ether phospholipids pl
dc.subject.en phosphatidylethanolamines pl
dc.subject.en Langmuir monolayers pl
dc.subject.en Brewster angle microscopy pl
dc.description.volume 97 pl
dc.identifier.doi 10.1016/j.colsurfb.2012.04.023 pl
dc.identifier.eissn 1873-4367 pl
dc.title.journal Colloids and Surfaces. B, Biointerfaces pl
dc.language.container eng pl
dc.affiliation Wydział Chemii : Zakład Chemii Ogólnej pl
dc.affiliation Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii pl
dc.affiliation Wydział Chemii : Zakład Chemii Środowiska pl
dc.subtype Article pl
dc.rights.original bez licencji pl
.pointsMNiSW [2012 A]: 30


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