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Spectroscopic and microscopic examination of protein adsorption and blocking of non-specific binding to silicon surfaces modified with APTES and GOPS

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Spectroscopic and microscopic examination of protein adsorption and blocking of non-specific binding to silicon surfaces modified with APTES and GOPS

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dc.contributor.author Awsiuk, Kamil [SAP14001148] pl
dc.contributor.author Psarouli, A. pl
dc.contributor.author Petrou, P. pl
dc.contributor.author Budkowski, Andrzej [SAP11011875] pl
dc.contributor.author Kakabakos, S. pl
dc.contributor.author Bernasik, A. pl
dc.contributor.author Rysz, Jakub [SAP11116295] pl
dc.contributor.author Raptis, I. pl
dc.date.accessioned 2016-11-23T12:22:34Z
dc.date.available 2016-11-23T12:22:34Z
dc.date.issued 2011 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/32758
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode *
dc.title Spectroscopic and microscopic examination of protein adsorption and blocking of non-specific binding to silicon surfaces modified with APTES and GOPS pl
dc.type JournalArticle pl
dc.description.physical 334-337 pl
dc.abstract.en X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM) and Time of Flight Secondary Ion Mass Spectrometry (TOF-SIMS) were applied to characterize two series of silicon nitride surfaces, modified either with (3-aminopropyl)triethoxysilane (APTES) or (3-glycidoxypropyl)trimethoxysilane (GOPS), prior to and after immobilization of rabbit gamma globulins (rIgG) at different concentrations and blocking with bovine serum albumin (BSA). Higher rIgG amount was adsorbed to surfaces silanized with APTES rather than GOPS, resulting in different behavior to subsequent blocking. There was no increase in total protein surface density due to blocking with BSA for surfaces with already high protein coverage. Apparently, BSA molecules were partly exchanged with rIgG ones, in case of APTES and higher rIgG concentrations, or attached to free surface sites, for GOPS modified surfaces. pl
dc.subject.en protein adsorption pl
dc.subject.en APTES pl
dc.subject.en GOPS pl
dc.subject.en TOF-SIMS pl
dc.subject.en AFM pl
dc.subject.en XPS pl
dc.description.volume 25 pl
dc.description.conftype international pl
dc.identifier.doi 10.1016/j.proeng.2011.12.082 pl
dc.identifier.eissn 1877-7058 pl
dc.title.journal Procedia Engineering pl
dc.title.volume EurosensorsXXV pl
dc.language.container eng pl
dc.affiliation Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego pl
dc.subtype ConferenceProceedings pl
dc.conference EurosensorsXXV; 2011-09-04; 2011-09-07; Ateny; Grecja; indeksowana w Web of Science; indeksowana w Scopus; ; pl
dc.rights.original CC-BY-NC-ND; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl


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Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0