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Externalization of phosphatidylserine from inner to outer layer may alter the effect of plant sterols on human erythrocyte membrane : the Langmuir monolayer studies

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Externalization of phosphatidylserine from inner to outer layer may alter the effect of plant sterols on human erythrocyte membrane : the Langmuir monolayer studies

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dc.contributor.author Hąc-Wydro, Katarzyna [SAP11018762] pl
dc.contributor.author Dynarowicz-Łątka, Patrycja [SAP11012997] pl
dc.date.accessioned 2015-02-20T12:45:19Z
dc.date.available 2015-02-20T12:45:19Z
dc.date.issued 2012 pl
dc.identifier.issn 0005-2736 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/3254
dc.language eng pl
dc.title Externalization of phosphatidylserine from inner to outer layer may alter the effect of plant sterols on human erythrocyte membrane : the Langmuir monolayer studies pl
dc.type JournalArticle pl
dc.description.physical 2184-2191 pl
dc.abstract.en One of the factors, which can strongly modify the cell membrane composition, is disordering in membrane asymmetry, resulting from redistribution of lipids from inner to outer layer. Such a disturbance may affect the behavior of various biologically active compounds incorporating into membranes. In this contribution, the relationship between the amounts of phosphatidylserine (PS) in the model outer layer of human eryth- rocyte (RBC) membrane and the effect induced by a plant sterol ( β -sitosterol) was veri fi ed. The experiments were performed on multicomponent Langmuir fi lms imitating red blood cell (RBC) membrane, differing in the contents of PS (0%; 5% and 10%) into which the plant sterol was incorporated in various concentrations. The analysis of experimental results (surface pressure – area isotherms complemented with Brewster Angle Microscopy (BAM) proved that the presence of phosphatidylserine molecules, depending on their contents in the mixed monolayer mimicking RBC membrane, changes its properties and exerts in fl uence on the effect of plant sterol on the model system. The addition of phytosterol into the monolayer that lacks or contains only 5% of PS was found to be of rather weak effect on the properties of the system. However, in the case of the model membrane containing the increased amount (10%) of PS, the incorporation of plant sterol strongly affects the interactions between molecules and caused thermodynamic destabilization of the mono- layer imitating RBC membrane. These results allow one to suggest that externalization of phosphatidyserine to the outer membrane lea fl et may differentiate the effect of plant sterols on cell membranes of various origins pl
dc.subject.en model outer layer of human erythrocyte pl
dc.subject.en langmuir film pl
dc.subject.en (RBC) membrane pl
dc.subject.en Brewster angle microscopy pl
dc.subject.en plant sterol pl
dc.subject.en Phosphatidylserine pl
dc.description.volume 1818 pl
dc.description.number 9 pl
dc.identifier.doi 10.1016/j.bbamem.2012.05.010 pl
dc.identifier.eissn 1879-2642 pl
dc.title.journal Biochimica et Biophysica Acta. Biomembranes pl
dc.language.container eng pl
dc.affiliation Wydział Chemii : Zakład Chemii Ogólnej pl
dc.affiliation Wydział Chemii : Zakład Chemii Środowiska pl
dc.subtype Article pl
dc.rights.original OTHER; otwarte czasopismo; ostateczna wersja wydawcy; po opublikowaniu; 12 pl
.pointsMNiSW [2012 A]: 35


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