Repozytorium Uniwersytetu Jagiellońskiego
Externalization of phosphatidylserine from inner to outer layer may alter the effect of plant sterols on human erythrocyte membrane : the Langmuir monolayer studies
Externalization of phosphatidylserine from inner to ...
Opis pozycji
| tytuł: | Externalization of phosphatidylserine from inner to outer layer may alter the effect of plant sterols on human erythrocyte membrane : the Langmuir monolayer studies |
| autor: | Hąc-Wydro Katarzyna  , Dynarowicz-Łątka Patrycja
|
| tytuł czasopisma: | Biochimica et Biophysica Acta. Biomembranes |
| tom: | 1818 |
| numer: | 9 |
| data wydania: | 2012 |
| strony: | 2184-2191 |
| ISSN: |
0005-2736 |
| eISSN: |
1879-2642 |
| DOI: | |
| język: | angielski |
| język czasopisma: | angielski |
| abstrakt w j. angielskim: | One of the factors, which can strongly modify the cell membrane composition, is disordering in membrane asymmetry, resulting from redistribution of lipids from inner to outer layer. Such a disturbance may affect the behavior of various biologically active compounds incorporating into membranes. In this contribution, the relationship between the amounts of phosphatidylserine (PS) in the model outer layer of human eryth- rocyte (RBC) membrane and the effect induced by a plant sterol ( β -sitosterol) was veri fi ed. The experiments were performed on multicomponent Langmuir fi lms imitating red blood cell (RBC) membrane, differing in the contents of PS (0%; 5% and 10%) into which the plant sterol was incorporated in various concentrations. The analysis of experimental results (surface pressure – area isotherms complemented with Brewster Angle Microscopy (BAM) proved that the presence of phosphatidylserine molecules, depending on their contents in the mixed monolayer mimicking RBC membrane, changes its properties and exerts in fl uence on the effect of plant sterol on the model system. The addition of phytosterol into the monolayer that lacks or contains only 5% of PS was found to be of rather weak effect on the properties of the system. However, in the case of the model membrane containing the increased amount (10%) of PS, the incorporation of plant sterol strongly affects the interactions between molecules and caused thermodynamic destabilization of the mono- layer imitating RBC membrane. These results allow one to suggest that externalization of phosphatidyserine to the outer membrane lea fl et may differentiate the effect of plant sterols on cell membranes of various origins |
| słowa kluczowe w j. angielskim: | model outer layer of human erythrocyte, langmuir film, (RBC) membrane, Brewster angle microscopy, plant sterol, Phosphatidylserine |
| wydział:instytut/zakład/katedra: | Wydział Chemii : Zakład Chemii Ogólnej, Wydział Chemii : Zakład Chemii Środowiska |
| typ: | artykuł w czasopiśmie |
| podtyp: | artykuł |
Pliki tej pozycji
| Plik | Rozmiar | Format | Przeglądanie |
|---|---|---|---|
|
Nie ma plików powiązanych z tą pozycją. |
|||