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Red blood cells polarize green laser light revealing hemoglobin's enhanced non-fundamental Raman modes

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Red blood cells polarize green laser light revealing hemoglobin's enhanced non-fundamental Raman modes

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dc.contributor.author Marzec, Katarzyna M. [SAP14003458] pl
dc.contributor.author Perez-Guaita, David pl
dc.contributor.author de Veij, Marleen pl
dc.contributor.author Barańska, Małgorzata [SAP11016906] pl
dc.contributor.author Dixon, Matthew W. A. pl
dc.contributor.author Tilley, Leann pl
dc.contributor.author Wood, Bayden R. pl
dc.contributor.author McNaughton, Don pl
dc.date.accessioned 2015-02-20T10:38:53Z
dc.date.available 2015-02-20T10:38:53Z
dc.date.issued 2014 pl
dc.identifier.issn 1439-4235 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/3248
dc.language eng pl
dc.rights Dodaję tylko opis bibliograficzny *
dc.rights.uri *
dc.title Red blood cells polarize green laser light revealing hemoglobin's enhanced non-fundamental Raman modes pl
dc.type JournalArticle pl
dc.description.physical 3963-3968 pl
dc.abstract.en In general, the first overtone modes produce weak bands that appear at approximately twice the wavenumber value of the fundamental transitions in vibrational spectra. Here, we report the existence of a series of enhanced non-fundamental bands in resonance Raman (RR) spectra recorded for hemoglobin (Hb) inside the highly concentrated heme environment of the red blood cell (RBC) by exciting with a 514.5 nm laser line. Such bands are most intense when detecting parallel-polarized light. The enhancement is explained through excitonic theory invoking a type C scattering mechanism and bands have been assigned to overtone and combination bands based on symmetry arguments and polarization measurements. By using malaria diagnosis as an example, we demonstrate that combining the non-fundamental and fundamental regions of the RR spectrum improves the sensitivity and diagnostic capability of the technique. The discovery will have considerable implications for the ongoing development of Raman spectroscopy for blood disease diagnoses and monitoring heme perturbation in response to environmental stimuli. pl
dc.subject.en malaria diagnostic pl
dc.subject.en overtone/combination modes pl
dc.subject.en porphyrinoids pl
dc.subject.en Raman spectroscopy pl
dc.subject.en red blood cells pl
dc.description.volume 15 pl
dc.description.number 18 pl
dc.description.points 35 pl
dc.identifier.doi 10.1002/cphc.201402598 pl
dc.identifier.eissn 1439-7641 pl
dc.title.journal ChemPhysChem pl
dc.language.container eng pl
dc.affiliation Wydział Chemii : Zakład Fizyki Chemicznej pl
dc.affiliation Pion Rektora : Jagiellońskie Centrum Rozwoju Leków pl
dc.subtype Article pl
dc.rights.original bez licencji pl
.pointsMNiSW [2014 A]: 35


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