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Preparation of tissue samples for X-ray fluorescence microscopy

Preparation of tissue samples for X-ray fluorescence ...

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dc.contributor.author Chwiej, Joanna pl
dc.contributor.author Szczerbowska-Boruchowska, Magdalena pl
dc.contributor.author Lankosz, Marek pl
dc.contributor.author Wójcik, Sławomir pl
dc.contributor.author Falkenberg, Gerald pl
dc.contributor.author Stęgowski, Zdzisław pl
dc.contributor.author Setkowicz-Janeczko, Zuzanna [SAP11017135] pl
dc.date.accessioned 2016-11-10T13:20:46Z
dc.date.available 2016-11-10T13:20:46Z
dc.date.issued 2005 pl
dc.identifier.issn 0584-8547 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/32277
dc.language eng pl
dc.rights Dodaję tylko opis bibliograficzny *
dc.rights.uri *
dc.title Preparation of tissue samples for X-ray fluorescence microscopy pl
dc.type JournalArticle pl
dc.description.physical 1531-1537 pl
dc.description.additional Zuzanna Setkowicz-Janeczko podpisana: Zuzanna Setkowicz pl
dc.abstract.en As is well-known, trace elements, especially metals, play an important role in the pathogenesis of many disorders. The topographic and quantitative elemental analysis of pathologically changed tissues may shed some new light on processes leading to the degeneration of cells in the case of selected diseases. An ideal and powerful tool for such purpose is the Synchrotron Microbeam X-ray Fluorescence technique. It enables the carrying out of investigations of the elemental composition of tissues even at the single cell level. The tissue samples for histopathological investigations are routinely fixed and embedded in paraffin. The authors try to verify the usefulness of such prepared tissue sections for elemental analysis with the use of X-ray fluorescence microscopy. Studies were performed on rat brain samples. Changes in elemental composition caused by fixation in formalin or paraformaldehyde and embedding in paraffin were examined. Measurements were carried out at the bending magnet beamline L of the Hamburger Synchrotronstrahlungslabor HASYLAB in Hamburg. The decrease in mass per unit area of K, Br and the increase in P, S, Fe, Cu and Zn in the tissue were observed as a result of the fixation. For the samples embedded in paraffin, a lower level of most elements was observed. Additionally, for these samples, changes in the composition of some elements were not uniform for different analyzed areas of rat brain. pl
dc.description.volume 60 pl
dc.description.number 12 pl
dc.identifier.doi 10.1016/j.sab.2005.10.002 pl
dc.identifier.eissn 1873-3565 pl
dc.title.journal Spectrochimica Acta. Part B, Atomic Spectroscopy pl
dc.language.container eng pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Zoologii pl
dc.subtype Article pl
dc.rights.original bez licencji pl

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