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Preparation of tissue samples for X-ray fluorescence microscopy
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Preparation of tissue samples for X-ray fluorescence microscopy
Bibliographic description
| title: | Preparation of tissue samples for X-ray fluorescence microscopy |
| author: | Chwiej Joanna, Szczerbowska-Boruchowska Magdalena, Lankosz Marek, Wójcik Sławomir, Falkenberg Gerald, Stęgowski Zdzisław, Setkowicz-Janeczko Zuzanna 
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| journal title: | Spectrochimica Acta. Part B, Atomic Spectroscopy |
| volume: | 60 |
| issue: | 12 |
| date of publication : | 2005 |
| pages: | 1531-1537 |
| ISSN: |
0584-8547 |
| eISSN: |
1873-3565 |
| DOI: | |
| notes: | Zuzanna Setkowicz-Janeczko podpisana: Zuzanna Setkowicz |
| language: | English |
| journal language: | English |
| abstract in English: | As is well-known, trace elements, especially metals, play an important role in the pathogenesis of many disorders. The topographic and quantitative elemental analysis of pathologically changed tissues may shed some new light on processes leading to the degeneration of cells in the case of selected diseases. An ideal and powerful tool for such purpose is the Synchrotron Microbeam X-ray Fluorescence technique. It enables the carrying out of investigations of the elemental composition of tissues even at the single cell level. The tissue samples for histopathological investigations are routinely fixed and embedded in paraffin. The authors try to verify the usefulness of such prepared tissue sections for elemental analysis with the use of X-ray fluorescence microscopy. Studies were performed on rat brain samples. Changes in elemental composition caused by fixation in formalin or paraformaldehyde and embedding in paraffin were examined. Measurements were carried out at the bending magnet beamline L of the Hamburger Synchrotronstrahlungslabor HASYLAB in Hamburg. The decrease in mass per unit area of K, Br and the increase in P, S, Fe, Cu and Zn in the tissue were observed as a result of the fixation. For the samples embedded in paraffin, a lower level of most elements was observed. Additionally, for these samples, changes in the composition of some elements were not uniform for different analyzed areas of rat brain. |
| affiliation: | Wydział Biologii i Nauk o Ziemi : Instytut Zoologii |
| type: | journal article |
| subtype: | academic paper |
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Projekt „Repozytorium otwartego dostępu do dorobku naukowego i dydaktycznego UJ” współfinansowany w ramach poddziałania 2.3.1 „Cyfrowe udostępnianie zasobów nauki” Programu Operacyjnego Polska Cyfrowa z Europejskiego Funduszu Rozwoju Regionalnego i budżetu państwa na podstawie umowy o dofinansowanie nr POPC.02.03.01-00-0030/17-00
