Intralesional injection of interferon gamma affects reactive proliferation of astrocytes in the neonatal rat brain : a preliminary study of the age-dependent effect

Intralesional injection of interferon gamma affects reactive proliferation of astrocytes in the neonatal rat brain : a preliminary study of the age-dependent effect

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Intralesional injection of interferon gamma affects reactive proliferation of astrocytes in the neonatal rat brain : a preliminary study of the age-dependent effect

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title:	Intralesional injection of interferon gamma affects reactive proliferation of astrocytes in the neonatal rat brain : a preliminary study of the age-dependent effect
author:	Pawliński Rafał, Setkowicz-Janeczko Zuzanna , Ziaja Marek , Sołtys Zbigniew , Janeczko Krzysztof
journal title:	Folia Histochemica et Cytobiologica
volume:	37
issue:	4
date of publication :	1999
pages:	237-242
ISSN:	0239-8508
eISSN:	1897-5631
notes:	Zuzanna Setkowicz-Janeczko podpisana nazwiskiem Setkowicz
language:	English
journal language:	English
abstract in English:	Following a mechanical lesion of the left cerebral hemisphere, newborn male rats received a single injection of recombinant rat interferon gamma (IFN gamma) into the lesion cavity at doses of 5, 50 and 500 U. One or two days after the injury the rats were injected with H-3-thymidine. Brain sections were immunostained for glial fibrillary acidic protein (GFAP), subjected to autoradiography and examined microscopically to record proliferating GFAP-immunopositive (GFAP+) astrocytes labeled with H-3-thymidine. Following the intermediate 50 U dose of IFN gamma, numbers of GFAP+ astrocytes and of their mitoses on day 1 after injury were significantly higher than in controls. Nevertheless, the astrocyte labeling index remained at the control level. Injections of the minimal 5 U or the maximal 500 U doses of IFN gamma had no effect on that day. On day 2, however, each of the three doses evoked a statistically significant but dose-independent reduction of the labeling index without similar changes in total number of GFAP+ astrocytes or in numbers of their divisions. The changes appear to indicate a non-linear relation between the intensity of reactive behaviour of astrocytes and the amount of IFN gamma injected into the lesion area. On the basis of previous publications, IFN gamma effects on the astrocyte reactivity are discussed as being age-dependent.
affiliation:	Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
type:	journal article
subtype:	academic paper

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