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The influence of microinjection parameters on cell survival and procedure efficiency
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Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
The influence of microinjection parameters on cell survival and procedure efficiency
Bibliographic description
| title: | The influence of microinjection parameters on cell survival and procedure efficiency |
| author: | Hajduk Joanna  , Szajna Konrad , Lisowski Bartosz , Rajfur Zenon
|
| journal title: | MethodsX |
| volume: | 10 |
| date of publication : | 2023 |
| pages: | 102107 |
| article ID: | 102107 |
| ISSN: |
2215-0161 |
| eISSN: |
2215-0161 |
| DOI: | |
| language: | English |
| journal language: | English |
| abstract in English: | Microinjection is a method commonly used to deliver various substances into cells. The procedure is performed on a widefield microscope stage using fine glass needle to penetrate the cell membrane. Microinjection can be carried out using a manual or semi-automatic mode. For commercially available equipment currently reported microinjection success rate and cell viability are relatively low (around 50% for both indicators). Here, for the first time, we systematically show how the microinjection effectiveness and cell viability are influenced by needle diameter and chosen microinjection mode. We found that manual mode entailed a higher injection rate, reducing cell viability at the same time. The reduction in needle diameter caused a significant increase in cell survival rate (from 43 to 73% for manual mode and from 58% to 86% for semi-automatic mode) and did not affect significantly the success rate. Our findings will help optimize this method in the context of cell biology research. |
| keywords in English: | microinjection, manual, semi-automatic, cell culture, fibroblasts |
| affiliation: | Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego, Wydział Farmaceutyczny : Zakład Farmacji Społecznej, Szkoła Doktorska Nauk Ścisłych i Przyrodniczych |
| type: | journal article |
| subtype: | academic paper |
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Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
Projekt „Repozytorium otwartego dostępu do dorobku naukowego i dydaktycznego UJ” współfinansowany w ramach poddziałania 2.3.1 „Cyfrowe udostępnianie zasobów nauki” Programu Operacyjnego Polska Cyfrowa z Europejskiego Funduszu Rozwoju Regionalnego i budżetu państwa na podstawie umowy o dofinansowanie nr POPC.02.03.01-00-0030/17-00
