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Adsorption of components of the plasma kinin-forming system on the surface of Porphyromonas gingivalis involves gingipains as the major docking platforms

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Adsorption of components of the plasma kinin-forming system on the surface of Porphyromonas gingivalis involves gingipains as the major docking platforms

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dc.contributor.author Rąpała-Kozik, Maria [SAP11012697] pl
dc.contributor.author Braś, Grażyna [SAP14000003] pl
dc.contributor.author Chruścicka, Barbara pl
dc.contributor.author Karkowska-Kuleta, Justyna [SAP13036970] pl
dc.contributor.author Sroka, Aneta [SAP12117031] pl
dc.contributor.author Herwald, Heiko pl
dc.contributor.author Nguyen, Ky-Anh pl
dc.contributor.author Eick, Sigrun pl
dc.contributor.author Potempa, Jan [SAP11010833] pl
dc.contributor.author Kozik, Andrzej [SAP11010989] pl
dc.date.accessioned 2015-02-13T07:34:57Z
dc.date.available 2015-02-13T07:34:57Z
dc.date.created 2011 pl
dc.date.issued 2010 pl
dc.identifier.issn 0019-9567 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/3064
dc.language eng pl
dc.rights Dodaję tylko opis bibliograficzny *
dc.rights.uri *
dc.title Adsorption of components of the plasma kinin-forming system on the surface of Porphyromonas gingivalis involves gingipains as the major docking platforms pl
dc.type JournalArticle pl
dc.description.physical 797-805 pl
dc.abstract.en Enhanced production of proinflammatory bradykinin-related peptides, the kinins, has been suggested to contribute to the pathogenesis of periodontitis, a common inflammatory disease of human gingival tissues. In this report, we describe a plausible mechanism of activation of the kinin-generating system, also known as the contact system or kininogen-kallikrein-kinin system, by the adsorption of its plasma-derived components such as high-molecular-mass kininogen (HK), prekallikrein (PK), and Hageman factor (FXII) to the cell surface of periodontal pathogen Porphyromonas gingivalis. The adsorption characteristics of mutant strains deficient in selected proteins of the cell envelope suggested that the surface-associated cysteine proteinases, gingipains, bearing hemagglutinin/adhesin domains (RgpA and Kgp) serve as the major platforms for HK and FXII adhesion. These interactions were confirmed by direct binding tests using microplate-immobilized gingipains and biotinylated contact factors. Other bacterial cell surface components such as fimbriae and lipopolysaccharide were also found to contribute to the binding of contact factors, particularly PK. Analysis of kinin release in plasma upon contact with P. gingivalis showed that the bacterial surface-dependent mechanism is complementary to the previously described kinin generation system dependent on HK and PK proteolytic activation by the gingipains. We also found that several P. gingivalis clinical isolates differed in the relative significance of these two mechanisms of kinin production. Taken together, these data show the importance of this specific type of bacterial surface-host homeostatic system interaction in periodontal infections. pl
dc.description.volume 79 pl
dc.description.number 2 pl
dc.identifier.doi 10.1128/IAI.00966-10 pl
dc.identifier.eissn 1098-5522 pl
dc.title.journal Infection and Immunity pl
dc.language.container eng pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Analitycznej pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Mikrobiologii pl
dc.subtype Article pl
dc.rights.original OTHER; otwarte czasopismo; ostateczna wersja wydawcy; po opublikowaniu; 6; pl
.pointsMNiSW [2010 A]: 32


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