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Rhodamine 6G conjugated to gold nanoparticles as labels for both SERS and fluorescence studies on live endothelial cells

Rhodamine 6G conjugated to gold nanoparticles as ...

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dc.contributor.author Jaworska, Aleksandra [SAP14002928] pl
dc.contributor.author Wójcik, Tomasz [SAP14005849] pl
dc.contributor.author Małek, Kamilla [SAP11018144] pl
dc.contributor.author Kwolek, Urszula [SAP14006777] pl
dc.contributor.author Kępczyński, Mariusz [SAP11015411] pl
dc.contributor.author Ansary, Abu A. pl
dc.contributor.author Chłopicki, Stefan [SAP14004240] pl
dc.contributor.author Barańska, Małgorzata [SAP11016906] pl
dc.date.accessioned 2015-02-11T14:58:02Z
dc.date.available 2015-02-11T14:58:02Z
dc.date.issued 2015 pl
dc.identifier.issn 0026-3672 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/3033
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Rhodamine 6G conjugated to gold nanoparticles as labels for both SERS and fluorescence studies on live endothelial cells pl
dc.type JournalArticle pl
dc.description.physical 119-127 pl
dc.identifier.weblink https://link.springer.com/content/pdf/10.1007%2Fs00604-014-1307-5.pdf pl
dc.abstract.en Fluorescence and surface-enhanced Raman scattering (SERS) spectroscopy were employed to investigate the cellular uptake of rhodamine 6G (R6G) alone and of R6G loaded with gold nanoparticles (AuNPs) by endothelial cells. R6G plays the role of a Raman reporter in SERS but also displays strong fluorescence. The presence of bare R6G molecules and R6G-AuNPs in the cytoplasm of the cells is detected via the 2D fluorescence of the dye after a 0.5 h of the incubation with R6G and R6G-AuNPs, and then the concentration of the dye increases within 4 h of exposure. The examination of the cellular uptake of the R6G and R6G-AuNPs species at different temperatures suggests that the internalization of the R6G-AuNPs into endothelial cells occurs mainly via endocytosis. 3D fluorescence imaging of R6G inside cells reveals inhomogeneous distribution of the dye in the cytoplasm. The SERS signal of the Raman reporter inside the cell disappears after 2 h of incubation with R6G-AuNPs and then amino acid residues, purines and pyrimidines become SERS-active via their interactions with the gold. The results highlight the significance of using multiple techniques to cover a spectrum of issues in the application of SERS nanosensors for probing an intracellular environment under comparable and standardized conditions. pl
dc.subject.en endothelium pl
dc.subject.en SERS imaging pl
dc.subject.en 2D and 3D fluorescence pl
dc.subject.en rhodamine 6G pl
dc.subject.en cellular uptake pl
dc.description.volume 182 pl
dc.description.number 1-2 pl
dc.description.points 35 pl
dc.identifier.doi 10.1007/s00604-014-1307-5 pl
dc.identifier.eissn 1436-5073 pl
dc.title.journal Microchimica Acta pl
dc.language.container eng pl
dc.date.accession 2019-02-15 pl
dc.affiliation Wydział Chemii : Zakład Fizyki Chemicznej pl
dc.affiliation Pion Rektora : Jagiellońskie Centrum Rozwoju Leków pl
dc.affiliation Wydział Lekarski : Zakład Farmakologii Doświadczalnej pl
dc.affiliation Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii pl
dc.subtype Article pl
dc.rights.original CC-BY; inne; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
dc.pbn.affiliation USOS78024:UJ.WCh; pl
.pointsMNiSW [2015 A]: 35

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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa