Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary

doi:10.1016/j.reprotox.2012.06.009

Activation of the enzymes of phase I (CYP2B1/2) and ...

Bibliographic description

punktacja MNiSW [2012 A]: 35

title:	Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary
author:	Karpeta Anna , Warzecha Katarzyna, Jerzak Justyna, Ptak Anna , Gregoraszczuk Ewa
Journal title:	Reproductive Toxicology
volume:	34
issue:	3
date of publication:	2012
pages:	436-442
ISSN:	0890-6238
eISSN:	1873-1708
DOI:	10.1016/j.reprotox.2012.06.009
URL:	http://www.sciencedirect.com/science/article/pii/S0890623812002353
date accessed:	2015-04-13
language:	English
Journal language:	English
abstract in english:	The aim of the current study was to determine metabolism of polybrominated diphenyl ether (BDE-47) in the porcine ovary. We analyzed the activity and expression of enzymes involved in phase I (CYP1A1 and CYP2B1/2) and phase II (SULT1A and COMT) of BDE-47 metabolism. Basal CYP1A1 and CYP2B1/2 activity increased during culture. BDE-47 had no effect on CYP1A1, however increased CYP2B1/2 activity after exposure for 6 h. Basal SULT1A activity was 2.5 fold lower than that of COMT, and both proteins were stable during culture. BDE-47 increased SULT1A after exposure for 6 h, and COMT activity after exposure for 24 and 48 h. BDE-47 had no effect on the expression of all investigated enzymes. In conclusion, fast activation of CYP2B1/2 and late activation of COMT (with a very low basal SULT1A activity) indicates a possible action of locally produced hydroxylated metabolites prior to their detoxification.
keywords in english:	BDE-47, CYP1A1, CYP2B1/2, SULT1A, COMT, ovary
affiliation:	Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
type:	journal article
subtype:	academic paper