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Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary
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Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary
Bibliographic description
| title: | Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary |
| author: | Karpeta Anna  , Warzecha Katarzyna, Jerzak Justyna, Ptak Anna , Gregoraszczuk Ewa
|
| journal title: | Reproductive Toxicology |
| volume: | 34 |
| issue: | 3 |
| date of publication : | 2012 |
| pages: | 436-442 |
| ISSN: |
0890-6238 |
| eISSN: |
1873-1708 |
| DOI: | |
| URL: |
http://www.sciencedirect.com/science/article/pii/S0890623812002353 |
| date accessed: |
2015-04-13 |
| language: | English |
| journal language: | English |
| abstract in English: | The aim of the current study was to determine metabolism of polybrominated diphenyl ether (BDE-47) in the porcine ovary. We analyzed the activity and expression of enzymes involved in phase I (CYP1A1 and CYP2B1/2) and phase II (SULT1A and COMT) of BDE-47 metabolism. Basal CYP1A1 and CYP2B1/2 activity increased during culture. BDE-47 had no effect on CYP1A1, however increased CYP2B1/2 activity after exposure for 6 h. Basal SULT1A activity was 2.5 fold lower than that of COMT, and both proteins were stable during culture. BDE-47 increased SULT1A after exposure for 6 h, and COMT activity after exposure for 24 and 48 h. BDE-47 had no effect on the expression of all investigated enzymes. In conclusion, fast activation of CYP2B1/2 and late activation of COMT (with a very low basal SULT1A activity) indicates a possible action of locally produced hydroxylated metabolites prior to their detoxification. |
| keywords in English: | BDE-47, CYP1A1, CYP2B1/2, SULT1A, COMT, ovary |
| affiliation: | Wydział Biologii i Nauk o Ziemi : Instytut Zoologii |
| type: | journal article |
| subtype: | academic paper |
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Projekt „Repozytorium otwartego dostępu do dorobku naukowego i dydaktycznego UJ” współfinansowany w ramach poddziałania 2.3.1 „Cyfrowe udostępnianie zasobów nauki” Programu Operacyjnego Polska Cyfrowa z Europejskiego Funduszu Rozwoju Regionalnego i budżetu państwa na podstawie umowy o dofinansowanie nr POPC.02.03.01-00-0030/17-00
