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Progress of elemental anomalies of hippocampal formation in the pilocarpine model of temporal lobe epilepsy-an X-ray fluorescence microscopy study

Progress of elemental anomalies of hippocampal ...

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dc.contributor.author Chwiej, Joanna pl
dc.contributor.author Kutorasińska, Justyna pl
dc.contributor.author Janeczko, Krzysztof [SAP11008688] pl
dc.contributor.author Gzieło, Kinga [SAP14002533] pl
dc.contributor.author Uram, Łukasz [USOS12754] pl
dc.contributor.author Appel, Karen pl
dc.contributor.author Simon, Rolf pl
dc.contributor.author Setkowicz-Janeczko, Zuzanna [SAP11017135] pl
dc.date.accessioned 2015-02-09T08:54:52Z
dc.date.available 2015-02-09T08:54:52Z
dc.date.issued 2012 pl
dc.identifier.issn 1618-2642 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/2888
dc.language eng pl
dc.title Progress of elemental anomalies of hippocampal formation in the pilocarpine model of temporal lobe epilepsy-an X-ray fluorescence microscopy study pl
dc.type JournalArticle pl
dc.description.physical 3071-3080 pl
dc.description.additional Zuzanna Setkowicz-Janeczko podpisana nazwiskiem Setkowicz, Kinga Gzieło podpisana nazwiskiem Gzieło-Jurek pl
dc.abstract.en In the present paper, X-ray fluorescence microscopy was applied to follow the processes occurring in rat hippocampal formation during the post-seizure period. In the study, one of the status epilepticus animal models of epilepsy was used, namely the model of temporal lobe epilepsy with pilocarpine-induced seizures. In order to analyze the dynamics of seizure-induced elemental changes, the samples taken from seizure-experiencing animals 3 h and 1, 4, and 7 days after proconvulsive agent administration were analyzed. The obtained results confirmed the utility of X-ray fluorescence microscopy in the research of mechanisms involved in the pathogenesis and progress of epilepsy. The topographic and quantitative elemental analysis of hippocampal formations from different periods of epileptogenesis showed that excitotoxicity, mossy fibers sprouting, and iron-induced oxidative stress may be the processes responsible for seizure-induced neurodegenerative changes and spontaneous recurrent seizures occurring in the chronic phase of the pilocarpine model. The analysis of correlations between the recorded elemental anomalies and quantitative parameters describing animal behavior in the acute period of pilocarpine-induced status epilepticus showed that the areal densities of selected elements measured in the latent period strongly depend on the progress of the acute phase. Especially important seem to be the observations done for Ca and Zn levels which suggest that the intensity of the pathological processes such as excitotoxicity and mossy fibers sprouting depend on the total time of seizure activity. These results as well as dependencies found between the levels of S, K, and Cu and the intensity of maximal seizures clearly confirm how important it is to control the duration and intensity of seizures in clinical practice. pl
dc.subject.en pilocarpine model of epilepsy pl
dc.subject.en topographic and quantitative elemental analysis pl
dc.subject.en X-ray fluorescence microscopy pl
dc.subject.en synchrotron radiation pl
dc.description.volume 404 pl
dc.description.number 10 pl
dc.identifier.doi 10.1007/s00216-012-6425-5 pl
dc.identifier.eissn 1618-2650 pl
dc.title.journal Analytical and Bioanalytical Chemistry pl
dc.language.container eng pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Zoologii pl
dc.subtype Article pl
.pointsMNiSW [2012 A]: 35


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