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In vitro response of human pathological hematopoietic cells to fludarabine phosphate

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In vitro response of human pathological hematopoietic cells to fludarabine phosphate

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dc.contributor.author Opydo-Chanek, Małgorzata [SAP11018603] pl
dc.contributor.author Stojak, Marta [USOS12738] pl
dc.contributor.author Mazur, Lidia [SAP11007113] pl
dc.date.accessioned 2016-06-10T11:11:49Z
dc.date.available 2016-06-10T11:11:49Z
dc.date.issued 2012 pl
dc.identifier.issn 0001-530X pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/27850
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode *
dc.title In vitro response of human pathological hematopoietic cells to fludarabine phosphate pl
dc.type JournalArticle pl
dc.description.physical 33-38 pl
dc.identifier.weblink http://journals.pan.pl/dlibra/publication/94633/edition/81654/content pl
dc.abstract.en The present study was undertaken to determine a possible influence of fludarabine (fludarabine phosphate, F-ara-AMP) on the cell viability and count. The experiments were performed in vitro on human acute lymphoblastic MOLT-4 cells, human acute myeloblastic ML-1 cells, and human histiocytic lymphoma U-937 cells. The research was conducted using the spectrophotometric and Beckman Coulter methods. The cell viability was analyzed using MTT assay. The cell count was detected using an electronic Z2 Coulter counter. Temporary changes in the cell viability and count were assessed at 24h and 48h after F-ara-AMP application. The in vitro activity of fludarabine phosphate against MOLT-4, ML-1, and U-937 cells was compared. F-ara-AMP applied at the four concentrations - 250 nM, 500 nM, 750 nM, and 1 μM - distinctly decreased the viability and count of the pathological hematopoietic cells. The effects of F-ara-AMP on MOLT-4, ML-1, and U-937 cells were dependent on the tested agent and its dose, the time intervals after the agent application, and the cell line used. ML-1 and U-937 cells appeared to be more resistant than MOLT-4 cells to the action of fludarabine phosphate. The in vitro response of the three human pathological hematopoietic cell lines to the F-ara-AMP action, was shown. pl
dc.subject.en Human pathological hematopoietic cells pl
dc.subject.en fludarabine phosphate pl
dc.subject.en in vitro cell response pl
dc.description.volume 54 pl
dc.title.journal Acta Biologica Cracoviensia. Series Zoologia pl
dc.language.container eng pl
dc.date.accession 2018-01-30 pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Zoologii pl
dc.subtype Article pl
dc.rights.original CC-BY-NC-ND; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0 pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2012 B]: 5


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Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0