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The present study was undertaken to determine a possible influence of fludarabine (fludarabine phosphate,
F-ara-AMP) on the cell viability and count. The experiments were performed in vitro on human acute lymphoblastic
MOLT-4 cells, human acute myeloblastic ML-1 cells, and human histiocytic lymphoma U-937 cells. The research
was conducted using the spectrophotometric and Beckman Coulter methods. The cell viability was analyzed using
MTT assay. The cell count was detected using an electronic Z2 Coulter counter. Temporary changes in the cell viability
and count were assessed at 24h and 48h after F-ara-AMP application. The in vitro activity of fludarabine phosphate
against MOLT-4, ML-1, and U-937 cells was compared. F-ara-AMP applied at the four concentrations - 250
nM, 500 nM, 750 nM, and 1 μM - distinctly decreased the viability and count of the pathological hematopoietic cells.
The effects of F-ara-AMP on MOLT-4, ML-1, and U-937 cells were dependent on the tested agent and its dose, the
time intervals after the agent application, and the cell line used. ML-1 and U-937 cells appeared to be more resistant
than MOLT-4 cells to the action of fludarabine phosphate. The in vitro response of the three human pathological
hematopoietic cell lines to the F-ara-AMP action, was shown.
słowa kluczowe w j. angielskim:
Human pathological hematopoietic cells, fludarabine phosphate, in vitro cell response
wydział: instytut / zakład / katedra:
Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
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