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Clonal structure and characterization of Staphylococcus aureus strains from invasive infections in paediatric patients from South Poland : association between age, spa types, clonal complexes, and genetic markers

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Clonal structure and characterization of Staphylococcus aureus strains from invasive infections in paediatric patients from South Poland : association between age, spa types, clonal complexes, and genetic markers

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dc.contributor.author Ilczyszyn, Weronika [USOS38585] pl
dc.contributor.author Sabat, Artur J. pl
dc.contributor.author Akkerboom, Viktoria pl
dc.contributor.author Szkarlat, Anna pl
dc.contributor.author Klepacka, Joanna pl
dc.contributor.author Sowa-Sierant, Iwona pl
dc.contributor.author Wasik, Barbara pl
dc.contributor.author Kosecka-Strojek, Maja [SAP14008834] pl
dc.contributor.author Buda, Aneta [USOS133515] pl
dc.contributor.author Międzobrodzki, Jacek [SAP11012289] pl
dc.contributor.author Friedrich, Alexander W. pl
dc.date.accessioned 2016-05-24T09:55:29Z
dc.date.available 2016-05-24T09:55:29Z
dc.date.issued 2016 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/26898
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Clonal structure and characterization of Staphylococcus aureus strains from invasive infections in paediatric patients from South Poland : association between age, spa types, clonal complexes, and genetic markers pl
dc.type JournalArticle pl
dc.description.additional W art. jest Weronika M. Ilczyszyn pl
dc.abstract.en The aim of current study was to examine clonal structure and genetic profile of invasive Staphylococcus aureus isolates recovered from infants and children treated at the Jagiellonian University Children’s Hospital of Krakow, Poland. The 107 invasive S. aureus isolates, collected between February 2012 and August 2014, were analysed retrospectively. Antimicrobial susceptibility testing, spa typing and DNA microarray analysis were performed to determine clonal distribution, diversity and gene content in regard to patients characteristics. In total, 107 isolates were recovered from 88 patients with clinical symptoms of invasive bacterial infection. The final set of 92 non-duplicate samples included 38 MRSA isolates. Additionally, a set of 54 S. aureus isolates collected during epidemiological screening was genotyped and analysed. There were 72 healthcare-associated (HCA) and 20 community-onset (CO) infection events caused by 33 and 5 MRSA isolates, respectively. The majority of isolates were affiliated with the major European clonal complexes CC5 (t003, spa-CC 002), CC45 (spa-CC 015), CC7 or CC15 (t084, t091, spa-CC 084). Two epidemic clones (CC5-MRSA-II or CC45-MRSA-IV) dominated among MRSA isolates, while MSSA population contained 15 different CCs. The epidemiological screening isolates belonged to similar genetic lineages as those collected from invasive infection cases. The HCA infection events, spa types t003, t2642 or CC5 were significantly associated with infections occurring in neonates and children under 5 years of age. Moreover, carriage of several genetic markers, including erm(A), sea (N315), egc-cluster, chp was significantly higher in isolates obtained from children in this age group. The spa types t091 and t008 were underrepresented among patients aged 5 years or younger, whereas spa type t008, CC8 and presence of splE was associated with infection in children aged 10 years or older. The HCA-MRSA strains were most frequently found in children under 5 years, although the majority of invasive infections was associated with MSSA strains. Moreover, an association between age group of children from the study population and a specific strain genotype (spa type, clonal complex or genetic content) was observed among the patients. pl
dc.description.volume 11 pl
dc.description.number 3 pl
dc.identifier.doi 10.1371/journal.pone.0151937 pl
dc.identifier.eissn 1932-6203 pl
dc.title.journal PLoS ONE pl
dc.language.container eng pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Mikrobiologii pl
dc.subtype Article pl
dc.identifier.articleid e0151937 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2016 A]: 35


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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa