Jagiellonian University Repository

Use of sensitive, broad-spectrum molecular assays and human airway epithelium cultures for detection of respiratory pathogens

pcg.skipToMenu

Use of sensitive, broad-spectrum molecular assays and human airway epithelium cultures for detection of respiratory pathogens

Show full item record

dc.contributor.author Pyrć, Krzysztof [SAP11019543] pl
dc.contributor.author Stożek, Karol [SAP14000407] pl
dc.contributor.author Wójcik, Krzysztof [SAP20011960] pl
dc.contributor.author Gawron, Katarzyna [SAP14002828] pl
dc.contributor.author Zeglen, Slawomir pl
dc.contributor.author Karolak, Wojciech pl
dc.contributor.author Wojarski, Jacek pl
dc.contributor.author Ochman, Marek pl
dc.contributor.author Hubalewska-Mazgaj, Magdalena [SAP20013114] pl
dc.contributor.author Bochenek, Grażyna [SAP20000240] pl
dc.contributor.author Sanak, Marek [SAP20001048] pl
dc.contributor.author Zembala, Marian pl
dc.contributor.author Szczeklik, Andrzej [SAP20000226] pl
dc.contributor.author Potempa, Jan [SAP11010833] pl
dc.date.accessioned 2016-05-18T12:49:18Z
dc.date.available 2016-05-18T12:49:18Z
dc.date.issued 2012 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/26399
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Use of sensitive, broad-spectrum molecular assays and human airway epithelium cultures for detection of respiratory pathogens pl
dc.type JournalArticle pl
dc.abstract.en Rapid and accurate detection and identification of viruses causing respiratory tract infections is important for patient care and disease control. Despite the fact that several assays are available, identification of an etiological agent is not possible in ∼30% of patients suffering from respiratory tract diseases. Therefore, the aim of the current study was to develop a diagnostic set for the detection of respiratory viruses with sensitivity as low as 1–10 copies per reaction. Evaluation of the assay using a training clinical sample set showed that viral nucleic acids were identified in ∼76% of cases. To improve assay performance and facilitate the identification of novel species or emerging strains, cultures of fully differentiated human airway epithelium were used to pre-amplify infectious viruses. This additional step resulted in the detection of pathogens in all samples tested. Based on these results it can be hypothesized that the lack of an etiological agent in some clinical samples, both reported previously and observed in the present study, may result not only from the presence of unknown viral species, but also from imperfections in the detection methods used. pl
dc.description.volume 7 pl
dc.description.number 3 pl
dc.identifier.doi 10.1371/journal.pone.0032582 pl
dc.identifier.eissn 1932-6203 pl
dc.title.journal PLoS ONE pl
dc.language.container eng pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Mikrobiologii pl
dc.affiliation Wydział Lekarski : Klinika Alergii i Immunologii pl
dc.affiliation Wydział Lekarski : Zakład Biologii Molekularnej i Genetyki Klinicznej pl
dc.affiliation Wydział Lekarski : Klinika Pulmonologii pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizyki Komórki pl
dc.subtype Article pl
dc.identifier.articleid e32582 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2012 A]: 40


Files in this item

This item appears in the following Collection(s)

Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa