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Heme oxygenase-1 regulates the progression of K/BxN serum transfer arthritis

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Heme oxygenase-1 regulates the progression of K/BxN serum transfer arthritis

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dc.contributor.author Brines, Rita pl
dc.contributor.author Maicas, Nuria pl
dc.contributor.author Ferrándiz, María Luisa pl
dc.contributor.author Łoboda, Agnieszka [SAP11019077] pl
dc.contributor.author Józkowicz, Alicja [SAP11114690] pl
dc.contributor.author Dulak, Józef [SAP11012135] pl
dc.contributor.author Alcaraz, María José pl
dc.date.accessioned 2016-05-17T15:52:21Z
dc.date.available 2016-05-17T15:52:21Z
dc.date.issued 2012 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/26256
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Heme oxygenase-1 regulates the progression of K/BxN serum transfer arthritis pl
dc.type JournalArticle pl
dc.abstract.en Heme oxygenase-1 (HO-1) is induced in many cell types as a defense mechanism against stress. We have investigated the possible role of endogenous HO-1 in the effector phase of arthritis using the K/BxN serum transfer model of arthritis in HO-1 heterozygous and homozygous knock-out mice. Methodology/Principal Findings. Arthritis was induced in C57/Black-6 xFVB (HO-1+/+, HO-1+/− and HO-1−/−) mice by intraperitoneal injection of 150 µl serum from arthritic K/BxN mice at days 0 and 2. Blood was collected and animals were sacrificed at day 10. Histological analysis was performed in ankle sections. The levels of inflammatory mediators were measured in serum and paw homogenates by enzyme-linked immunosorbent assay or Multiplex technology. The incidence of arthritis was higher in HO-1+/− and HO-1−/− groups compared with HO-1+/+. The inflammatory response was aggravated in HO-1+/− mice as shown by arthritic score and the migration of inflammatory cells that could be related to the enhancement of CXCL-1 production. In addition, the HO-1+/− group showed proteoglycan depletion significantly higher than HO-1+/+ mice. Serum levels of matrix metalloproteinase-3, monocyte chemotactic protein-1, plasminogen activator inhibitor-1, E-selectin and intercellular adhesion molecule-1 were increased in arthritic HO-1−/− mice, whereas vascular endothelial growth factor and some cytokines such as interferon-γ showed a reduction compared to HO-1+/+ or HO-1+/− mice. In addition, down-regulated gene expression of ferritin, glutathione S-reductase A1 and superoxide dismutase-2 was observed in the livers of arthritic HO-1+/− animals. pl
dc.description.volume 7 pl
dc.description.number 12 pl
dc.identifier.doi 10.1371/journal.pone.0052435 pl
dc.identifier.eissn 1932-6203 pl
dc.title.journal PLoS ONE pl
dc.language.container eng pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biotechnologii Medycznej pl
dc.subtype Article pl
dc.identifier.articleid e52435 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2012 A]: 40


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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa