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Synchrotron FTIR micro-spectroscopy study of the rat hippocampal formation after pilocarpine-evoked seizures


Synchrotron FTIR micro-spectroscopy study of the rat hippocampal formation after pilocarpine-evoked seizures

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dc.contributor.author Chwiej, Joanna pl
dc.contributor.author Dulińska, Justyna pl
dc.contributor.author Janeczko, Krzysztof [SAP11008688] pl
dc.contributor.author Dumas, Paul pl
dc.contributor.author Eichert, Diane pl
dc.contributor.author Dudała, Joanna pl
dc.contributor.author Setkowicz-Janeczko, Zuzanna [SAP11017135] pl
dc.date.accessioned 2015-01-16T11:56:25Z
dc.date.available 2015-01-16T11:56:25Z
dc.date.issued 2010 pl
dc.identifier.issn 0891-0618 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/2582
dc.language eng pl
dc.rights Dodaję tylko opis bibliograficzny *
dc.rights.uri *
dc.title Synchrotron FTIR micro-spectroscopy study of the rat hippocampal formation after pilocarpine-evoked seizures pl
dc.type JournalArticle pl
dc.description.physical 140-147 pl
dc.description.additional Zuzanna Setkowicz-Janeczko podpisana nazwiskiem Setkowicz pl
dc.abstract.en In the present work, synchrotron radiation Fourier transform infrared (SRFTIR) micro-spectroscopy and imaging were used for topographic and semi-quantitative biochemical analysis of rat brain tissue in cases of pilocarpine-induced epilepsy. The tissue samples were analyzed with a beam defined by small apertures and spatial resolution steps of 10 mu m which allowed us to probe the selected cellular layers of hippocampal formation. Raster scanning of the samples has generated 2D chemical cartographies revealing the distribution of proteins, lipids and nucleic acids. Spectral analysis has shown changes in the saturation level of phospholipids and relative secondary structure of proteins. Special interest was put in the analysis of two areas of the hippocampal formation (sector 3 of the Ammon's horn, CA3 and dentate gyrus, DG) in which elemental abnormalities were observed during our previous studies. Statistically significant increase in the saturation level of phospholipids (increased ratio of the absorption intensities at around 2921 and 2958 cm(-1)) as well as conformational changes of proteins (beta-type structure discrepancies as shown by the increased ratio of the absorbance intensities at around 1631 and 1657 cm(-1) as well as the ratio of the absorbance at 1548 and 1657 cm(-1)) were detected in pyramidal cells of CA3 area as well as in the multiform and molecular layers of DG. The findings presented here suggest that abnormalities in the protein secondary structure and increases in the level of phospholipid saturation could be involved in mechanisms of neurodegenerative changes following the oxidative stress evoked in brain areas affected by pilocarpine-induced seizures. pl
dc.subject.en pilocarpine-induced epilepsy pl
dc.subject.en SRFTIR micro-spectroscopy and imaging pl
dc.subject.en biochemical analysis pl
dc.subject.en lipid peroxidation pl
dc.subject.en relative secondary structure of proteins pl
dc.description.volume 40 pl
dc.description.number 2 pl
dc.identifier.doi 10.1016/j.jchemneu.2010.03.008 pl
dc.identifier.eissn 1873-6300 pl
dc.title.journal Journal of Chemical Neuroanatomy pl
dc.language.container eng pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Zoologii pl
dc.subtype Article pl
.pointsMNiSW [2010 A]: 27

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