Jagiellonian University Repository

Elasticity changes anti-correlate with NO production for human endothelial cells stimulated with TNF-$\alpha$

pcg.skipToMenu

Elasticity changes anti-correlate with NO production for human endothelial cells stimulated with TNF-$\alpha$

Show full item record

dc.contributor.author Szczygiel, Agnieszka M. pl
dc.contributor.author Brzezinka, Grzegorz [SAP14002606] pl
dc.contributor.author Targosz-Korecka, Marta [SAP11019208] pl
dc.contributor.author Chłopicki, Stefan [SAP14004240] pl
dc.contributor.author Szymoński, Marek [SAP11006649] pl
dc.date.accessioned 2016-05-10T09:26:39Z
dc.date.available 2016-05-10T09:26:39Z
dc.date.issued 2012 pl
dc.identifier.issn 0031-6768 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/25238
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne 3.0 Polska *
dc.rights.uri http://creativecommons.org/licenses/by-nc/3.0/pl/legalcode *
dc.title Elasticity changes anti-correlate with NO production for human endothelial cells stimulated with TNF-$\alpha$ pl
dc.type JournalArticle pl
dc.description.physical 487-496 pl
dc.abstract.en Tumor necrosis factor alpha (TNF-$\alpha$) is a critical cytokine that is involved in systemic inflammatory response and contributes to the activation of the pro-inflammatory phenotype of the endothelium. In the present study, effects of TNF-$\alpha$ on morphology and elasticity of endothelium in relation to the production of NO and actin fiber reorganization were analyzed in human dermal microvascular endothelial cells. The cells were incubated in MCDB medium solution and stimulated with 10ng/ml of TNF-$\alpha$. Atomic force microscopy measurements have enabled characterization of cell morphology and elastic properties in physiological conditions. The spectrophotometric Griess method was applied to estimate nitric oxide (NO) production of the cells. We demonstrated that TNF-$\alpha$-induced changes in elasticity of endothelium anti-correlate with NO production and are associated with the reorganization of actin cytoskeleton. pl
dc.subject.en endothelium pl
dc.subject.en tumor necrosis factor-$\alpha$ pl
dc.subject.en nitric oxide pl
dc.subject.en atomic force microscopy pl
dc.subject.en cytoskeleton pl
dc.description.volume 463 pl
dc.description.number 3 pl
dc.description.publication 0,6 pl
dc.identifier.doi 10.1007/s00424-011-1051-1 pl
dc.identifier.eissn 1432-2013 pl
dc.title.journal Pflügers Archiv pl
dc.language.container eng pl
dc.affiliation Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego pl
dc.affiliation Pion Rektora : Jagiellońskie Centrum Rozwoju Leków pl
dc.subtype Article pl
dc.rights.original CC-BY-NC; inne; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2012 A]: 30


Files in this item

This item appears in the following Collection(s)

Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne 3.0 Polska Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne 3.0 Polska