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Effect of histone deacetylase inhibitors trichostatin A and valproic acid on etoposide-induced apoptosis in leukemia cells

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Effect of histone deacetylase inhibitors trichostatin A and valproic acid on etoposide-induced apoptosis in leukemia cells

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dc.contributor.author Jasek-Gajda, Ewa [SAP20002508] pl
dc.contributor.author Lis, Grzegorz [SAP20001013] pl
dc.contributor.author Jasińska, Małgorzata [SAP20012228] pl
dc.contributor.author Jurkowska, Halina [SAP20002052] pl
dc.contributor.author Litwin, Jan [SAP20000544] pl
dc.date.accessioned 2020-10-20T09:02:59Z
dc.date.available 2020-10-20T09:02:59Z
dc.date.issued 2012 pl
dc.identifier.issn 0250-7005 pl
dc.identifier.uri https://ruj.uj.edu.pl/xmlui/handle/item/248424
dc.language eng pl
dc.rights Dozwolony użytek utworów chronionych *
dc.rights.uri http://ruj.uj.edu.pl/4dspace/License/copyright/licencja_copyright.pdf *
dc.title Effect of histone deacetylase inhibitors trichostatin A and valproic acid on etoposide-induced apoptosis in leukemia cells pl
dc.type JournalArticle pl
dc.description.physical 2791-2799 pl
dc.description.additional Bibliogr. s. 2798-2799 pl
dc.identifier.weblink http://ar.iiarjournals.org/content/32/7/2791.full.pdf+html pl
dc.abstract.en Background: Histone deacetylase inhibitors (HDACi) have been extensively studied as potential candidates for treatment of various malignancies, including leukemia, since they not only induce growth inhibition, cell cycle arrest and apoptosis of cancer cells, but can also increase the sensitivity of cancer cells to chemotherapeutic drugs. The aim of this study was to investigate the effect of two HDACi, trichostatin A (TSA) and valproic acid (VPA), on etoposide-induced apoptosis in human leukemia cell lines. Materials and Methods: Viability, apoptosis rate, caspase activity, mitochondrial membrane potential and expression of BCL2 mRNA were assessed in HL60 and U937 cell lines treated with 250 nM TSA or 1.25 mM VPA alone or followed by 5 μM etoposide. Results: Preincubation of HL60 cells with TSA or VPA significantly potentiated etoposide-induced cytotoxicity and apoptosis, which was associated with activation of caspases and loss of mitochondrial membrane potential. Similar effects were not observed in U937 cells. Expression of BCL2 mRNA was strongly down-regulated after treatment of cells with HDACi alone but did not show additive effect with etoposide. Conclusion: Combination of HDACi with etoposide can have a synergistic effect on increased apoptosis in leukemia cells but this effect depends on the cancer cell type and other factors such as the concentration of drugs and the administration schedule. pl
dc.subject.en HDACi pl
dc.subject.en etoposide pl
dc.subject.en leukemia pl
dc.subject.en apoptosis pl
dc.subject.en trichostatin A pl
dc.subject.en valproic acid pl
dc.subject.en HL60 pl
dc.subject.en U937 cells pl
dc.description.volume 32 pl
dc.description.number 7 pl
dc.description.publication 0,79 pl
dc.identifier.eissn 1791-7530 pl
dc.title.journal Anticancer Research pl
dc.language.container eng pl
dc.date.accession 2020-10-20 pl
dc.affiliation Wydział Lekarski : Zakład Histologii pl
dc.affiliation Wydział Lekarski : Zakład Biochemii Ogólnej pl
dc.subtype Article pl
dc.rights.original OTHER; otwarte czasopismo; ostateczna wersja wydawcy; po opublikowaniu; 24 pl
dc.identifier.project ROD UJ / OP pl
.pointsMNiSW [2012 A]: 20


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