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Substrate binding favors enhanced NO binding to
Ferric cytochrome
dc.abstract.en | Ferric cytochrome $P450_{cam}$ from Pseudomonas putida ($P450_{cam}$) in buffer solution at physiological pH 7.4 reversibly binds NO to yield the nitrosyl complex $P450_{cam}$(NO). The presence of 1R-camphor affects the dynamics of NO binding to $P450_{cam}$ and enhances the association and dissociation rate constants significantly. In the case of the substrate-free form of $P450_{cam}$, subconformers are evident and the NO binding kinetics are much slower than in the presence of the substrate. The association and dissociation processes were investigated by both laser flash photolysis and stopped-flow techniques at ambient and high pressure. Large and positive values of $\Delta$S⧧ and $\Delta$V⧧ observed for NO binding to and release from the substrate-free $P450_{cam}$ complex are consistent with the operation of a limiting dissociative ligand substitution mechanism, where the lability of coordinated water dominates the reactivity of the iron(III)-heme center with NO. In contrast, NO binding to $P450_{cam}$ in the presence of camphor displays negative activation entropy and activation volume values that support a mechanism dominated by a bond formation process. Volume profiles for the binding of NO appear to be a valuable approach to explain the differences observed for $P450_{cam}$ in the absence and presence of the substrate and enable the clarification of the underlying reaction mechanisms at a molecular level. Changes in spin state of the iron center during the binding/release of NO contribute significantly to the observed volume effects. The results are discussed in terms of relevance for the biological function of cytochrome P450 and in context to other investigations of the related reactions between NO and imidazole- and thiolate-ligated iron(III) hemoproteins. | pl |
dc.affiliation | Wydział Chemii : Zakład Chemii Nieorganicznej | pl |
dc.contributor.author | Franke, Alicja | pl |
dc.contributor.author | Stochel, Grażyna - 132108 | pl |
dc.contributor.author | Jung, Christiane | pl |
dc.contributor.author | van Eldik, Rudi - 239234 | pl |
dc.date.accessioned | 2015-01-02T11:50:21Z | |
dc.date.available | 2015-01-02T11:50:21Z | |
dc.date.issued | 2004 | pl |
dc.description.number | 13 | pl |
dc.description.physical | 4181-4191 | pl |
dc.description.volume | 126 | pl |
dc.identifier.doi | 10.1021/ja038774d | pl |
dc.identifier.eissn | 1520-5126 | pl |
dc.identifier.issn | 0002-7863 | pl |
dc.identifier.uri | http://ruj.uj.edu.pl/xmlui/handle/item/2479 | |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Dodaję tylko opis bibliograficzny | * |
dc.rights.licence | bez licencji | |
dc.rights.uri | * | |
dc.subtype | Article | pl |
dc.title | Substrate binding favors enhanced NO binding to $P450_{cam}$ | pl |
dc.title.journal | Journal of the American Chemical Society | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |