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Endothelial precursor cell-based therapy to target the pathologic angiogenesis and compensate tumor hypoxia

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Endothelial precursor cell-based therapy to target the pathologic angiogenesis and compensate tumor hypoxia

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dc.contributor.author Collet, Guillaume pl
dc.contributor.author Szade, Krzysztof [SAP14008846] pl
dc.contributor.author Nowak, Witold [SAP14008673] pl
dc.contributor.author Klimkiewicz, Krzysztof [USOS61086] pl
dc.contributor.author El Hafny-Rahbi, Bouchra pl
dc.contributor.author Szczepanek, Karol [USOS21044] pl
dc.contributor.author Sugiyama, Daisuke pl
dc.contributor.author Weglarczyk, Kazimierz pl
dc.contributor.author Foucault-Collet, Alexandra pl
dc.contributor.author Guichard, Alan pl
dc.contributor.author Mazan, Andrzej pl
dc.contributor.author Nadim, Mahdi pl
dc.contributor.author Fasani, Fabienne pl
dc.contributor.author Lamerant-Fayel, Nathalie pl
dc.contributor.author Grillon, Catherine pl
dc.contributor.author Petoud, Stéphane pl
dc.contributor.author Beloeil, Jean-Claude pl
dc.contributor.author Józkowicz, Alicja [SAP11114690] pl
dc.contributor.author Dulak, Józef [SAP11012135] pl
dc.contributor.author Kieda, Claudine [SAP14000889] pl
dc.date.accessioned 2016-04-11T08:54:44Z
dc.date.available 2016-04-11T08:54:44Z
dc.date.issued 2016 pl
dc.identifier.issn 0304-3835 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/23747
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/pl/legalcode *
dc.title Endothelial precursor cell-based therapy to target the pathologic angiogenesis and compensate tumor hypoxia pl
dc.type JournalArticle pl
dc.description.physical 345-357 pl
dc.abstract.en Hypoxia-inducing pathologies as cancer develop pathologic and inefficient angiogenesis which rules tumor facilitating microenvironment, a key target for therapy. As such, the putative ability of endothelial precursor cells (EPCs) to specifically home to hypoxic sites of neovascularization prompted to design optimized, site-specific, cell-mediated, drug-/gene-targeting approach. Thus, EPC lines were established from aorta–gonad–mesonephros (AGM) of murine 10.5 dpc and 11.5 dpc embryo when endothelial repertoire is completed. Lines representing early endothelial differentiation steps were selected: MAgEC10.5 and MagEC11.5. Distinct in maturation, they differently express VEGF receptors, VE-cadherin and chemokine/receptors. MAgEC11.5, more differentiated than MAgEC 10.5, displayed faster angiogenesis in vitro, different response to hypoxia and chemokines. Both MAgEC lines cooperated to tube-like formation with mature endothelial cells and invaded tumor spheroids through a vasculogenesis-like process. In vivo, both MAgEC-formed vessels established blood flow. Intravenously injected, both MAgECs invaded MatrigelTM-plugs and targeted tumors. Here we show that EPCs (MAgEC11.5) target tumor angiogenesis and allow local overexpression of hypoxia-driven soluble VEGF-receptor2 enabling drastic tumor growth reduction. We propose that such EPCs, able to target tumor angiogenesis, could act as therapeutic gene vehicles to inhibit tumor growth by vessel normalization resulting from tumor hypoxia alleviation. pl
dc.subject.en endothelial precursor cells pl
dc.subject.en pathologic angiogenesis/vasculogenesis pl
dc.subject.en tumor targeting pl
dc.subject.en cell gene therapy pl
dc.description.volume 370 pl
dc.description.number 2 pl
dc.identifier.doi 10.1016/j.canlet.2015.11.008 pl
dc.identifier.eissn 1872-7980 pl
dc.title.journal Cancer Letters pl
dc.language.container eng pl
dc.affiliation Pion Prorektora ds. badań naukowych i funduszy strukturalnych : Małopolskie Centrum Biotechnologii pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biotechnologii Medycznej pl
dc.subtype Article pl
dc.rights.original CC-BY-NC-ND; inne; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2016 A]: 35


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Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 4.0 Międzynarodowa