title:
|
Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy |
author: |
Kubicz Ewelina , Jasińska Bożena, Zgardzińska Bożena, Bednarski Tomasz , Białas Piotr , Czerwiński Eryk , Gajos Aleksander , Gorgol Marek, Kisielewska Daria , Kapłon Łukasz , Kochanowski Andrzej , Korcyl Grzegorz , Kowalski Paweł, Kozik Tomasz , Krzemień Wojciech , Niedźwiecki Szymon , Pałka Marek , Raczyński Lech, Rajfur Zenon , Rudy Zbigniew , Rundel Oleksandr , Gupta-Sharma Neha , Silarski Michał , Słomski Artur , Strzelecki Adam , Wieczorek Anna , Wiślicki Wojciech, Zieliński Marcin , Moskal Paweł
|
journal title:
|
Nukleonika |
title of volume:
|
Proceedings of the 42nd Polish Seminar on Positron Annihilation Lublin, Poland, 29 June – 1 July 2015 |
volume: |
60 |
issue:
|
4 |
date of publication
:
|
2015 |
pages:
|
749-753 |
ISSN: |
0029-5922
|
eISSN: |
1508-5791
|
DOI: |
10.1515/nuka-2015-0135
|
URL: |
http://www.nukleonika.pl/www/back/full/vol60_2015/v60n4p749f.pdf
|
date accessed: |
2016-03-17
|
notes:
|
Autorka Daria Kisielewska podpisana jako Daria Kamińska. |
language: |
English |
journal language:
|
English |
abstract in English: |
Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images. |
keywords in English: |
environmental scanning electron microscopy (ESEM), free volume, inverted microscopy, positron annihilation, positronium, yeasts |
number of pulisher's sheets: |
0,3 |
affiliation: |
Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego, Wydział Fizyki, Astronomii i Informatyki Stosowanej : Zakład Technologii Informatycznych, Wydział Fizyki, Astronomii i Informatyki Stosowanej : Zakład Technologii Gier, Wydział Chemii : Zakład Technologii Chemicznej |
type: |
journal article |
subtype: |
academic paper |
punktacja MEiN [2015 A]: 15