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Urinary hepcidin levels in iron-deficient and iron-supplemented piglets correlate with hepcidin hepatic mRNA and serum levels and with body iron status

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Urinary hepcidin levels in iron-deficient and iron-supplemented piglets correlate with hepcidin hepatic mRNA and serum levels and with body iron status

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dc.contributor.author Staroń, Robert pl
dc.contributor.author Van Swelm, Rachel P. L. pl
dc.contributor.author Lipiński, Paweł pl
dc.contributor.author Gajowiak, Anna pl
dc.contributor.author Lenartowicz, Małgorzata [SAP11012906] pl
dc.contributor.author Bednarz, Aleksandra [SAP14046483] pl
dc.contributor.author Gajewska, Małgorzata pl
dc.contributor.author Pieszka, Marek pl
dc.contributor.author Laarakkers, Coby M. M pl
dc.contributor.author Swinkels, Dorine W. pl
dc.contributor.author Starzyński, Rafał R. pl
dc.date.accessioned 2016-02-12T08:36:29Z
dc.date.available 2016-02-12T08:36:29Z
dc.date.issued 2015 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/20780
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Urinary hepcidin levels in iron-deficient and iron-supplemented piglets correlate with hepcidin hepatic mRNA and serum levels and with body iron status pl
dc.type JournalArticle pl
dc.abstract.en Among livestock, domestic pig (Sus scrofa) is a species, in which iron metabolism has been most intensively examined during last decade. The obvious reason for studying the regulation of iron homeostasis especially in young pigs is neonatal iron deficiency anemia commonly occurring in these animals. Moreover, supplementation of essentially all commercially reared piglets with iron entails a need for monitoring the efficacy of this routine practice followed in the swine industry for several decades. Since the discovery of hepcidin many studies confirmed its role as key regulator of iron metabolism and pointed out the assessment of its concentrations in biological fluids as diagnostic tool for iron-related disorder. Here we demonstrate that urine hepcidin-25 levels measured by a combination of weak cation exchange chromatography and time-of-flight mass spectrometry (WCX-TOF MS) are highly correlated with mRNA hepcidin expression in the liver and plasma hepcidin-25 concentrations in anemic and iron-supplemented 28-day old piglets. We also found a high correlation between urine hepcidin level and hepatic non-heme iron content. Our results show that similarly to previously described transgenic mouse models of iron disorders, young pigs constitute a convenient animal model to explore accuracy and relationship between indicators for assessing systemic iron status. pl
dc.description.volume 10 pl
dc.description.number 8 pl
dc.identifier.doi 10.1371/journal.pone.0136695 pl
dc.identifier.eissn 1932-6203 pl
dc.title.journal PLoS ONE pl
dc.language.container eng pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Zoologii pl
dc.subtype Article pl
dc.identifier.articleid e0136695 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2015 A]: 40


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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa