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Does long-term high fat diet always lead to smaller hippocampi volumes, metabolite concentrations, and worse learning and memory? : a magnetic resonance and behavioral study in wistar rats

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Does long-term high fat diet always lead to smaller hippocampi volumes, metabolite concentrations, and worse learning and memory? : a magnetic resonance and behavioral study in wistar rats

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dc.contributor.author Setkowicz-Janeczko, Zuzanna [SAP11017135] pl
dc.contributor.author Gaździńska, Agata pl
dc.contributor.author Osoba, Joanna [USOS38973] pl
dc.contributor.author Karwowska, Karolina [USOS112322] pl
dc.contributor.author Majka, Piotr pl
dc.contributor.author Orzeł, Jarosław pl
dc.contributor.author Kossowski, Bartosz pl
dc.contributor.author Bogorodzki, Piotr pl
dc.contributor.author Janeczko, Krzysztof [SAP11008688] pl
dc.contributor.author Wyleżoł, Mariusz pl
dc.contributor.author Gaździński, Stefan P. pl
dc.date.accessioned 2016-01-21T07:17:26Z
dc.date.available 2016-01-21T07:17:26Z
dc.date.issued 2015 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/19974
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Does long-term high fat diet always lead to smaller hippocampi volumes, metabolite concentrations, and worse learning and memory? : a magnetic resonance and behavioral study in wistar rats pl
dc.type JournalArticle pl
dc.description.additional Zuzanna Setkowicz-Janeczko podpisana: Zuzanna Setkowicz pl
dc.abstract.en Background. Obesity is a worldwide epidemic with more than 600 million affected individuals. Human studies have demonstrated some alterations in brains of otherwise healthy obese individuals and elevated risk of neurodegenerative disease of old age; these studies have also pointed to slightly diminished memory and executive functions among healthy obese individuals. Similar findings were obtained in animal models of obesity induced by high fat diet. On the other hand, low carbohydrate high fat diets are currently promoted for losing weight (e.g., Atkin’s style diets). However, the long-term effects of such diets are not known. Additionally, high fat diets leading to (mild) ketonemia were shown to improve brain function in elderly humans and in some animal models. Aim. To evaluate the hypothesis that long-term use of a high fat diet was associated with decreases in spatial memory, smaller hippocampi and hippocampi metabolite concentrations in Wistar rats. Methods. Twenty five male Wistar rats were put on high fat diet (HFD; 60% calories from fat, 30% from carbohydrates) on their 55th day of life, while 25 control male rats (CONs) remained on chow. Adequate levels of essential nutrients were provided. Both groups underwent memory tests in 8-arm radial maze at 3rd, 6th, 9th, and 12th month. 1H magnetic resonance spectroscopy was employed to measure concentrations of tNAA (marker of neuronal integrity) at one month and one year, whereas MRI was used to evaluate hippocampal volumes. Results. Obese rats (OBRs) consumed similar amount of calories as CONs, but less proteins. However, their protein intake was within recommended amounts. Throughout the experiment OBRs had statistically higher concentrations of blood ketone bodies than CONs, but still within normal values. At post-mortem assessment, OBRs had 38% larger fat deposits than CONs (p<0.05), as evaluated by volume of epididymis fat, an acknowledged marker of fat deposits in rats. Contrary to our expectations, OBRs had better scores of memory behavioral tasks than CONs throughout the experiment. At one year, their hippocampi were by 2.6% larger than in CONs (p = 0.05), whereas concentration of tNAA was 9.8% higher (p = 0.014). Conclusion. Long-term HFD in our study resulted in better memory, larger hippocampal volumes, as well as higher hippocampal metabolite concentrations, possibly due to increased levels of blood ketone bodies. The results should be interpreted with caution, as results from animal models do not necessarily directly translate in human condition. pl
dc.description.volume 10 pl
dc.description.number 10 pl
dc.identifier.doi 10.1371/journal.pone.0139987 pl
dc.identifier.eissn 1932-6203 pl
dc.title.journal PLoS ONE pl
dc.language.container eng pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Zoologii pl
dc.subtype Article pl
dc.identifier.articleid e0139987 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2015 A]: 40


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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa