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A three-marker DNA barcoding approach for ecological studies of xerothermic plants and herbivorous insects from central Europe

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A three-marker DNA barcoding approach for ecological studies of xerothermic plants and herbivorous insects from central Europe

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dc.contributor.author Heise, Waldemar [USOS87755] pl
dc.contributor.author Babik, Wiesław [SAP11018324] pl
dc.contributor.author Kubisz, Daniel pl
dc.contributor.author Kajtoch, Łukasz pl
dc.date.accessioned 2016-01-11T14:09:14Z
dc.date.available 2016-01-11T14:09:14Z
dc.date.issued 2015 pl
dc.identifier.issn 0024-4074 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/19208
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne *
dc.rights.uri https://creativecommons.org/licenses *
dc.title A three-marker DNA barcoding approach for ecological studies of xerothermic plants and herbivorous insects from central Europe pl
dc.type JournalArticle pl
dc.description.physical 576-592 pl
dc.abstract.en The DNA barcoding technique developed for species identification has recently been adapted for ecological studies (e.g. host plant identification). Comprehensive barcode databases, covering most species inhabiting areas, habitats or communities of interest are essential for reliable and efficient identification of plants. Here we present a three-barcode (plastid rbcL and matK genes and the trnL intron) database for xerothermic plant species from central Europe. About 85% of the xerothermic plant species (126 out of c. 150) known to be associated with xerothermic habitats were collected and barcoded. The database contains barcodes for 117 (rbcL and trnL) and 96 (matK) species. Interspecific nucleotide distances were in the ranges 0–17.9% (0–3.2% within genera) for rbcL, 0–44.4% (0–3.1%) for trnL and 0–52.5% (0–10.9%) for matK. Blast-searching of each sequence in the database against the entire database showed that species-level identification is possible for 89.6% (rbcL), 98.4% (trnL) and 96.4% (matK) of examined plant species. The utility of the presented database for identification of host plants was demonstrated using two insect species associated with xerothermic habitats: the oligophagous leaf-beetle Cheilotoma musciformis (for which two host plants in Fabaceae were identified) and the polyphagous weevil Polydrusus inustus (which was found to feed on 14 host plants, mostly Rosaceae, Asteraceae and Fabaceae). The developed database will be useful in various applications, including biodiversity, phylogeography, conservation and ecology pl
dc.subject.en calcareous grasslands pl
dc.subject.en Coleoptera pl
dc.subject.en dry grasslands pl
dc.subject.en matK pl
dc.subject.en plastid DNA pl
dc.subject.en rbcL pl
dc.subject.en trnL pl
dc.description.volume 177 pl
dc.description.number 4 pl
dc.identifier.doi 10.1111/boj.12261 pl
dc.identifier.eissn 1095-8339 pl
dc.title.journal Botanical Journal of the Linnean Society pl
dc.language.container eng pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Nauk o Środowisku pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Botaniki pl
dc.subtype Article pl
dc.rights.original CC-BY-NC; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2015 A]: 35


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Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne