Jagiellonian University Repository

Application of spin labels for research of vanadyl acetylacetonate concentration in model bilayer membranes by EPR spectroscopy

pcg.skipToMenu

Application of spin labels for research of vanadyl acetylacetonate concentration in model bilayer membranes by EPR spectroscopy

Show full item record

dc.contributor.author Hałas, Alicja pl
dc.contributor.author Sułkowska, Anna pl
dc.contributor.author Maślanka, Sławomir pl
dc.contributor.author Gryboś, Ryszard [SAP11007128] pl
dc.contributor.author Sułkowski, Wiesław W. pl
dc.date.accessioned 2016-01-04T07:59:02Z
dc.date.available 2016-01-04T07:59:02Z
dc.date.issued 2012 pl
dc.identifier.issn 1542-1406 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/18815
dc.language eng pl
dc.title Application of spin labels for research of vanadyl acetylacetonate concentration in model bilayer membranes by EPR spectroscopy pl
dc.type JournalArticle pl
dc.description.physical 195-208 pl
dc.abstract.en The compounds and complexes of vanadium are used to treat diabetes and cancer. Research on the effectiveness and mechanism of action of new derivatives of vanadium, and their toxicity is currently very intense. The research shows that the vanadium(IV) acetylacetonate complex [VO(acac)2] shows a synergism with insulin in treating diabetes, high pharmacological activity and low toxicity. In order to improve the effectiveness of drugs and minimize their toxicity, the active compounds are often closed in the liposome membranes. The objective of the work was preparation of bilayer liposomes from egg yolk phosphatidylcholine (EYPC), closing the complex VO(acac)2 in these membranes and estimating the concentration of vanadium complex after incorporation into liposomes membranes. Due to the paramagnetic properties of vanadium(IV) the concentration of this metal complex can be determined directly by EPR. Entering the spin label CTPO in the water phase into the studied arrangement allows for the indirect measurement of the concentration of complex, on the basis of changes of the EPR spectrum of the spin label caused by the presence of the vanadium(IV) complex. In the work the dependence of the α parameter based on the analysis of CTPO EPR spectra on the concentration of VO(acac)2 was determined. To demonstrate the presence of the complex in the membrane directly by measuring the EPR sulfate(IV) sodium was used in order to remove the EPR signal of vanadium(IV) from the water phase. The presence of vanadium(IV) in the membrane was also demonstrated indirectly using a spin label 12-SASL. Based on the results of EPR spectroscopy the concentration of the complex in the membrane was determined together with the partition coefficient of VO(acac)2 between the membrane and outer water environment of the membrane. pl
dc.subject.en concentration pl
dc.subject.en spin labels pl
dc.subject.en liposomes pl
dc.subject.en vanadium(IV) acetyloacetonate complex pl
dc.description.volume 554 pl
dc.description.number 1 pl
dc.description.points 15 pl
dc.identifier.doi 10.1080/15421406.2012.634321 pl
dc.identifier.eissn 1563-5287 pl
dc.title.journal Molecular Crystals and Liquid Crystals pl
dc.title.volume Special Issue: Proceedings of the 11th International Conference on Frontiers of Polymers and Advanced Materials (ICFPAM 2011) pl
dc.language.container eng pl
dc.affiliation Wydział Chemii : Zakład Chemii Nieorganicznej pl
dc.subtype Article pl
dc.rights.original bez licencji pl
.pointsMNiSW [2012 A]: 15


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)