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Cyclosporin A in membrane lipids environment : implications for antimalarial activity of the drug : the Langmuir monolayer studies

Cyclosporin A in membrane lipids environment : ...

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dc.contributor.author Dynarowicz-Łątka, Patrycja [SAP11012997] pl
dc.contributor.author Wnętrzak, Anita [SAP14013174] pl
dc.contributor.author Makyła-Juzak, Katarzyna [SAP14003871] pl
dc.date.accessioned 2015-12-14T14:32:11Z
dc.date.available 2015-12-14T14:32:11Z
dc.date.issued 2015 pl
dc.identifier.issn 0022-2631 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/18250
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Cyclosporin A in membrane lipids environment : implications for antimalarial activity of the drug : the Langmuir monolayer studies pl
dc.type JournalArticle pl
dc.description.physical 1021-1032 pl
dc.identifier.weblink https://link.springer.com/content/pdf/10.1007%2Fs00232-015-9814-9.pdf pl
dc.abstract.en Cyclosporin A (CsA), a hydrophobic cyclic peptide produced by the fungus Tolypocladium inflatum, is well known for its high efficiency as an immunosuppressor for transplanted organs and anti-inflammatory properties; however, it is also active as antiparasitic (antimalarial) drug. Antimalarial mechanism of CsA action lacks a detailed understanding at molecular level. Due to a high lipophilicity of CsA, it is able to interact with lipids of cellular membrane; however, molecular targets of this drug are still unknown. To get a deeper insight into the mode of antimalarial activity of CsA, it is of utmost importance to examine its interactions with membrane components. To reach this goal, the Langmuir monolayer technique, which serves as a very useful, easy to handle and controllable model of biomembranes, has been employed. In this work, the interactions between CsA and main membrane lipids, i.e., cholesterol (Chol), 2-oleoyl-1-palmitoyl-3-phosphocholine (POPC), and sphingomyelin (SM), have been investigated. Attractive interactions are observed only for CsA mixtures with SM, while repulsive forces occur in systems containing remaining membrane lipids. Taking into consideration mutual interactions between membrane lipids (Chol–SM; Chol–POPC and SM–POPC), the behavior of CsA in model erythrocyte membrane of normal and infected cells has been analyzed. Our results prove strong affinity of CsA to SM in membrane environment. Since normal and parasitized erythrocytes differ significantly in the level of SM, this phospholipid may be considered as a molecular target for antimalarial activity of CsA. pl
dc.subject.en Cyclosporin A pl
dc.subject.en interactions pl
dc.subject.en membrane lipids pl
dc.subject.en Langmuir monolayers pl
dc.subject.en model of normal and parasitized erythrocyte membrane pl
dc.description.volume 248 pl
dc.description.number 6 pl
dc.description.publication 0,7 pl
dc.identifier.doi 10.1007/s00232-015-9814-9 pl
dc.identifier.eissn 1432-1424 pl
dc.title.journal The Journal of Membrane Biology pl
dc.language.container eng pl
dc.date.accession 2019-02-15 pl
dc.affiliation Wydział Chemii : Zakład Chemii Ogólnej pl
dc.affiliation Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego pl
dc.subtype Article pl
dc.rights.original CC-BY; inne; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2015 A]: 20

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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa