5-HT_{7} receptor modulates GABAergic transmission in the rat dorsal raphe nucleus and controls cortical release of serotonin

doi:10.3389/fncel.2015.00324

5-HT_{7} receptor modulates GABAergic transmission in the rat dorsal raphe nucleus and controls cortical release of serotonin

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5-HT_{7} receptor modulates GABAergic transmission in the rat dorsal raphe nucleus and controls cortical release of serotonin

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dc.contributor.author	Kusek, Magdalena	pl
dc.contributor.author	Sowa, Joanna	pl
dc.contributor.author	Kamińska, Katarzyna	pl
dc.contributor.author	Gołembiowska, Krystyna	pl
dc.contributor.author	Tokarski, Krzysztof	pl
dc.contributor.author	Hess, Grzegorz [SAP11011175]	pl
dc.date.accessioned	2015-10-14T11:37:22Z
dc.date.available	2015-10-14T11:37:22Z
dc.date.issued	2015	pl
dc.identifier.uri	http://ruj.uj.edu.pl/xmlui/handle/item/16075
dc.language	eng	pl
dc.rights	Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/pl/legalcode	*
dc.title	5-HT_{7} receptor modulates GABAergic transmission in the rat dorsal raphe nucleus and controls cortical release of serotonin	pl
dc.type	JournalArticle	pl
dc.abstract.en	The 5-HT7 receptor is one of the several serotonin (5-HT) receptor subtypes that are expressed in the dorsal raphe nucleus (DRN). Some earlier findings suggested that 5-HT7 receptors in the DRN were localized on GABAergic interneurons modulating the activity of 5-HT projection neurons. The aim of the present study was to find out how the 5-HT7 receptor modulates the GABAergic synaptic input to putative 5-HT DRN neurons, and whether blockade of the 5-HT7 receptor would affect the release of 5-HT in the target structure. Male Wistar rats with microdialysis probes implanted in the prefrontal cortex (PFC) received injections of the 5-HT7 receptor antagonist (2R)-1-[(3-hydroxyphenyl)sulfonyl]-2-[2-(4-methyl-1-piperidinyl)ethyl]pyrrolidine hydrochloride (SB 269970), which induced an increase in the levels of 5-HT and its metabolite, 5-hydroxyindoleacetic acid (5-HIAA) in the PFC. In another set of experiments whole-cell recordings from presumed projection neurons were carried out using DRN slices. SB 269970 application resulted in depolarization and in an increase in the firing frequency of the cells. In order to activate 5-HT7 receptors, 5-carboxamidotryptamine (5-CT) was applied in the presence of N-[2-[4-(2-methoxyphenyl)-1piperazinyl]ethyl]-N-2-pyridinylcyclohexanecarboxamide (WAY100635). Hyperpolarization of cells and a decrease in the firing frequency were observed after activation of the 5-HT7 receptor. Blockade of 5-HT7 receptors caused a decrease in the mean frequency of spontaneous inhibitory postsynaptic currents (sIPSCs), while its activation induced an increase. The mechanism of these effects appears to involve tonically-active 5-HT7 receptors modulating firing and/or GABA release from inhibitory interneurons which regulate the activity of DRN serotonergic projection neurons.	pl
dc.subject.en	brain slices	pl
dc.subject.en	HPLC	pl
dc.subject.en	microdialysis	pl
dc.subject.en	prefrontal cortex	pl
dc.subject.en	whole-cell recording	pl
dc.description.volume	9	pl
dc.identifier.doi	10.3389/fncel.2015.00324	pl
dc.identifier.eissn	1662-5102	pl
dc.title.journal	Frontiers in Cellular Neuroscience	pl
dc.language.container	eng	pl
dc.affiliation	Wydział Biologii i Nauk o Ziemi : Instytut Zoologii	pl
dc.subtype	Article	pl
dc.identifier.articleid	324	pl
dc.rights.original	CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0;	pl
dc.identifier.project	ROD UJ / P	pl
.pointsMNiSW	[2015 A]: 35