The effects of short-term selenium stress on Polish and Finnish wheat seedlings : EPR, enzymatic and fluorescence studies

doi:10.1016/j.jplph.2011.10.012

The effects of short-term selenium stress on Polish ...

Bibliographic description

punktacja MNiSW [2012 A]: 35

title:	The effects of short-term selenium stress on Polish and Finnish wheat seedlings : EPR, enzymatic and fluorescence studies
author:	Łabanowska Maria , Filek Maria, Kościelniak Janusz, Kurdziel Magdalena , Kuliś Ewa , Hartikainen Helina
Journal title:	Journal of Plant Physiology
volume:	169
issue:	3
date of publication:	2012
pages:	275-284
ISSN:	0176-1617
eISSN:	1618-1328
DOI:	10.1016/j.jplph.2011.10.012
language:	English
Journal language:	English
abstract in english:	Biochemical analyses of antioxidant content were compared with measurements of fluorescence and electron paramagnetic resonance (EPR) to examine the alteration of radicals in wheat seedlings exposed to 2 days of selenium stress. Two genotypes of Polish and one of Finnish wheat, differing in their tol- erance to long-term stress treatment, were cultured under hydroponic conditions to achieve the phase of 3-leave seedlings. Afterwards, selenium (sodium selenate, 100 M concentration) was added to the media. After Se-treatment, all varieties showed an increase in carbohydrates (soluble and starch), ascor- bate and glutathione content in comparison to non-stressed plants. These changes were more visible in Finnish wheat. On the basis of lipid peroxidation measurements, Finnish wheat was recognized as the genotype more sensitive to short-term Se-stress than the Polish varieties. The antioxidant enzyme activities (superoxide dismutase, ascorbate peroxidase and glutathione reductase) increased in Polish genotypes, whereas they decreased in Finnish wheat plants cultured on Se media. The action of reactive oxygen species in short-term action of Se stress was confirmed by the reduction of PSII and PSI system activities (measured by fluorescence parameters and EPR, respectively). EPR studies showed changes in redox status (especially connected with Mn(II)/Mn(III), and semiquinone/quinone ratios) in wheat cell after Se treatment. The involvement of the carbohydrate molecules as electron traps in production of long-lived radicals is postulated.
keywords in english:	antioxidants, Fluorescence, EPR, Selenium, Stress tolerance, Whea
departmental parameterization:	35
affiliation:	Wydział Chemii : Zakład Chemii Nieorganicznej
type:	journal article
subtype:	academic paper