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Influence of nafion in titania sol-gel matrix on analytical characteristic of amperometric phenol biosensor based on tyrosinase

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Influence of nafion in titania sol-gel matrix on analytical characteristic of amperometric phenol biosensor based on tyrosinase

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dc.contributor.author Kochana, Jolanta [SAP11013864] pl
dc.date.accessioned 2015-09-03T07:38:48Z
dc.date.available 2015-09-03T07:38:48Z
dc.date.issued 2012 pl
dc.identifier.issn 1318-0207 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/15314
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Influence of nafion in titania sol-gel matrix on analytical characteristic of amperometric phenol biosensor based on tyrosinase pl
dc.type JournalArticle pl
dc.description.physical 760-765 pl
dc.identifier.weblink http://acta-arhiv.chem-soc.si/59/59-4-760.pdf pl
dc.abstract.en For detection of phenolic compounds a simple amperometric biosensor based on tyrosinase immobilized in titania sol- gel/Nafion composite was employed. Titania sol-gel was mixed with Nafion (v/v) in ratios 1:1 (TiO 2 /NF-1/1) and 2:1 (TiO 2 /NF-2/1), v/v. Morphology of immobilization composites was characterized by scanning electron microscopy. Analytical performance of biosensors with Nafion and based only on titania sol-gel (TiO 2 ) was compared. Apart from sensitivity, linear range and detection limit also repeatability, reproducibility and storage stability were evaluated. The biosensor based on titania sol mixed with Nafion in ratio 1:1 (v/v) exhibited the best analytical parameters in terms of sensitivity: 2.84 μA L μmol –1 , corresponding LOD, 0.056 μmol L –1 , and the long-term stability within 20 days: it re- tained 80% of initial activity. pl
dc.subject.en biosensor pl
dc.subject.en Nafion pl
dc.subject.en titania gel pl
dc.subject.en phenol pl
dc.subject.en tyrosinase pl
dc.description.volume 59 pl
dc.description.number 4 pl
dc.description.points 25 pl
dc.identifier.eissn 1580-3155 pl
dc.title.journal Acta Chimica Slovenica pl
dc.language.container eng pl
dc.date.accession 2015-09-03 pl
dc.affiliation Wydział Chemii : Zakład Chemii Analitycznej pl
dc.subtype Article pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0 pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2012 A]: 25


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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa