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Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell

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Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell

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dc.contributor.author Wróbel, Tomasz P. [USOS36247] pl
dc.contributor.author Marzec, Katarzyna M. [SAP14003458] pl
dc.contributor.author Majzner, Katarzyna [SAP14008951] pl
dc.contributor.author Kochan, Kamila [USOS80030] pl
dc.contributor.author Bartuś, Magdalena [SAP20002507] pl
dc.contributor.author Chłopicki, Stefan [SAP14004240] pl
dc.contributor.author Barańska, Małgorzata [SAP11016906] pl
dc.date.accessioned 2015-09-02T17:38:28Z
dc.date.available 2015-09-02T17:38:28Z
dc.date.issued 2012 pl
dc.identifier.issn 0003-2654 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/15305
dc.language eng pl
dc.title Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell pl
dc.type JournalArticle pl
dc.description.physical 4135-4139 pl
dc.abstract.en Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) with the use of a slide-on germanium accessory followed by chemometric analysis allowed for providing meaningful information about the biochemical composition of a single endo- thelial cell. In this work, the methodology of the ATR-FTIR measurements of dried cells and dried cells immersed in water solution is presented. The contact of the cell and Ge crystal was set up manually and monitored through the integration of the amide I band. Additionally, the cell imaging in transreflection mode was tested, but the spectral differences between sub-cellular structures were not prominent in the registered spectra. It has been shown that the ATR-FTIR method gives better results due to the increased spatial resolution and S/R ratio as well as small contribution of the optical artifacts pl
dc.description.volume 137 pl
dc.description.number 18 pl
dc.description.points 45 pl
dc.identifier.doi 10.1039/C2AN35331H pl
dc.identifier.eissn 1364-5528 pl
dc.title.journal Analyst pl
dc.language.container eng pl
dc.affiliation Wydział Chemii : Zakład Fizyki Chemicznej pl
dc.affiliation Pion Rektora : Jagiellońskie Centrum Rozwoju Leków pl
dc.affiliation Wydział Lekarski : Zakład Farmakologii Doświadczalnej pl
dc.affiliation Wydział Lekarski : Zakład Farmakologii pl
dc.subtype Article pl
dc.rights.original bez licencji pl
.pointsMNiSW [2012 A]: 45


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