Attenuated total reflection Fourier transform infrared spectroscopy
(ATR-FTIR) with the use of a slide-on germanium accessory
followed by chemometric analysis allowed for providing meaningful
information about the biochemical composition of a single endo-
thelial cell. In this work, the methodology of the ATR-FTIR
measurements of dried cells and dried cells immersed in water
solution is presented. The contact of the cell and Ge crystal was set
up manually and monitored through the integration of the amide I
band. Additionally, the cell imaging in transreflection mode was
tested, but the spectral differences between sub-cellular structures
were not prominent in the registered spectra. It has been shown that
the ATR-FTIR method gives better results due to the increased
spatial resolution and S/R ratio as well as small contribution of the
optical artifacts
departmental parameterization:
45
affiliation:
Wydział Chemii : Zakład Fizyki Chemicznej, Pion Rektora : Jagiellońskie Centrum Rozwoju Leków, Wydział Lekarski : Zakład Farmakologii Doświadczalnej, Wydział Lekarski : Zakład Farmakologii