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Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell
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Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell
Bibliographic description
| title: | Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell |
| author: | Wróbel Tomasz P.  , Marzec Katarzyna M. , Majzner Katarzyna , Kochan Kamila , Bartuś Magdalena , Chłopicki Stefan , Barańska Małgorzata
|
| journal title: | Analyst |
| volume: | 137 |
| issue: | 18 |
| date of publication : | 2012 |
| pages: | 4135-4139 |
| ISSN: |
0003-2654 |
| eISSN: |
1364-5528 |
| DOI: | |
| language: | English |
| journal language: | English |
| abstract in English: | Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) with the use of a slide-on germanium accessory followed by chemometric analysis allowed for providing meaningful information about the biochemical composition of a single endo- thelial cell. In this work, the methodology of the ATR-FTIR measurements of dried cells and dried cells immersed in water solution is presented. The contact of the cell and Ge crystal was set up manually and monitored through the integration of the amide I band. Additionally, the cell imaging in transreflection mode was tested, but the spectral differences between sub-cellular structures were not prominent in the registered spectra. It has been shown that the ATR-FTIR method gives better results due to the increased spatial resolution and S/R ratio as well as small contribution of the optical artifacts |
| departmental parameterization: | 45 |
| affiliation: | Wydział Chemii : Zakład Fizyki Chemicznej, Pion Rektora : Jagiellońskie Centrum Rozwoju Leków, Wydział Lekarski : Zakład Farmakologii Doświadczalnej, Wydział Lekarski : Zakład Farmakologii |
| type: | journal article |
| subtype: | academic paper |
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Projekt „Repozytorium otwartego dostępu do dorobku naukowego i dydaktycznego UJ” współfinansowany w ramach poddziałania 2.3.1 „Cyfrowe udostępnianie zasobów nauki” Programu Operacyjnego Polska Cyfrowa z Europejskiego Funduszu Rozwoju Regionalnego i budżetu państwa na podstawie umowy o dofinansowanie nr POPC.02.03.01-00-0030/17-00
