Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell

doi:10.1039/C2AN35331H

Attenuated total reflection Fourier transform infrared ...

Bibliographic description

punktacja MNiSW [2012 A]: 45

title:	Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell
author:	Wróbel Tomasz P. , Marzec Katarzyna M. , Majzner Katarzyna , Kochan Kamila , Bartuś Magdalena , Chłopicki Stefan , Barańska Małgorzata
Journal title:	Analyst
volume:	137
issue:	18
date of publication:	2012
pages:	4135-4139
ISSN:	0003-2654
eISSN:	1364-5528
DOI:	10.1039/C2AN35331H
language:	English
Journal language:	English
abstract in english:	Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) with the use of a slide-on germanium accessory followed by chemometric analysis allowed for providing meaningful information about the biochemical composition of a single endo- thelial cell. In this work, the methodology of the ATR-FTIR measurements of dried cells and dried cells immersed in water solution is presented. The contact of the cell and Ge crystal was set up manually and monitored through the integration of the amide I band. Additionally, the cell imaging in transreflection mode was tested, but the spectral differences between sub-cellular structures were not prominent in the registered spectra. It has been shown that the ATR-FTIR method gives better results due to the increased spatial resolution and S/R ratio as well as small contribution of the optical artifacts
departmental parameterization:	45
affiliation:	Wydział Chemii : Zakład Fizyki Chemicznej, Pion Rektora : Jagiellońskie Centrum Rozwoju Leków, Wydział Lekarski : Zakład Farmakologii Doświadczalnej, Wydział Lekarski : Zakład Farmakologii
type:	journal article
subtype:	academic paper