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Tyrosinase biosensor for benzoic acid inhibition-based determination with the use of a flow-batch monosegmented sequential injection system

Tyrosinase biosensor for benzoic acid inhibition-based ...

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dc.contributor.author Kochana, Jolanta [SAP11013864] pl
dc.contributor.author Kozak, Joanna [SAP11015410] pl
dc.contributor.author Skrobisz, Agata pl
dc.contributor.author Woźniakiewicz, Michał [SAP11019917] pl
dc.date.accessioned 2015-08-28T06:41:17Z
dc.date.available 2015-08-28T06:41:17Z
dc.date.issued 2012 pl
dc.identifier.issn 0039-9140 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/15121
dc.language eng pl
dc.title Tyrosinase biosensor for benzoic acid inhibition-based determination with the use of a flow-batch monosegmented sequential injection system pl
dc.type JournalArticle pl
dc.description.physical 147-152 pl
dc.abstract.en Amperometric tyrosinase based biosensor for benzoic acid determination with the use of a flow-batch monosegmented sequential injection system is proposed. The functioning of the biosensor is based on the inhibition effect of benzoic acid on the biocatalytic activity of tyrosinase, polyphenol oxidase. In the biosensor, tyrosinase is entrapped in titania gel modified with multiwalled carbon nanotubes (MWCNT) and Nafion. The procedure of optimization of experimental conditions affecting the biosensor response, as well as its analytical characteristics and results of the approach verification using synthetic samples and a reference material are presented. The developed biosensor exhibits linear range up to 2.46 M, good sensitivity toward benzoic acid (1.06 A M − 1 ) and low detection limit (0.03 M). The approach has been applied to benzoic acid determination in a real beverage sample giving the result consistent with that obtained using the HPLC method. pl
dc.subject.en benzoic acid pl
dc.subject.en inhibition pl
dc.subject.en sequential injection technique pl
dc.subject.en tyrosinase pl
dc.subject.en amperometry pl
dc.description.volume 96 pl
dc.description.points 40 pl
dc.identifier.doi 10.1016/j.talanta.2011.12.009 pl
dc.identifier.eissn 1873-3573 pl
dc.title.journal Talanta pl
dc.title.volume Selected Papers presented at the 17th ICFIA, Krakow, Poland (2011) pl
dc.language.container eng pl
dc.affiliation Wydział Chemii : Zakład Chemii Analitycznej pl
dc.subtype Article pl
dc.rights.original bez licencji pl
.pointsMNiSW [2012 A]: 40


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