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Tyrosinase biosensor for benzoic acid inhibition-based determination with the use of a flow-batch monosegmented sequential injection system
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Tyrosinase biosensor for benzoic acid inhibition-based determination with the use of a flow-batch monosegmented sequential injection system
Bibliographic description
| title: | Tyrosinase biosensor for benzoic acid inhibition-based determination with the use of a flow-batch monosegmented sequential injection system |
| author: | Kochana Jolanta  , Kozak Joanna , Skrobisz Agata, Woźniakiewicz Michał
|
| journal title: | Talanta |
| title of volume: | Selected Papers presented at the 17th ICFIA, Krakow, Poland (2011) |
| volume: | 96 |
| date of publication : | 2012 |
| pages: | 147-152 |
| ISSN: |
0039-9140 |
| eISSN: |
1873-3573 |
| DOI: | |
| language: | English |
| journal language: | English |
| abstract in English: | Amperometric tyrosinase based biosensor for benzoic acid determination with the use of a flow-batch monosegmented sequential injection system is proposed. The functioning of the biosensor is based on the inhibition effect of benzoic acid on the biocatalytic activity of tyrosinase, polyphenol oxidase. In the biosensor, tyrosinase is entrapped in titania gel modified with multiwalled carbon nanotubes (MWCNT) and Nafion. The procedure of optimization of experimental conditions affecting the biosensor response, as well as its analytical characteristics and results of the approach verification using synthetic samples and a reference material are presented. The developed biosensor exhibits linear range up to 2.46 M, good sensitivity toward benzoic acid (1.06 A M − 1 ) and low detection limit (0.03 M). The approach has been applied to benzoic acid determination in a real beverage sample giving the result consistent with that obtained using the HPLC method. |
| keywords in English: | benzoic acid, inhibition, sequential injection technique, tyrosinase, amperometry |
| departmental parameterization: | 40 |
| affiliation: | Wydział Chemii : Zakład Chemii Analitycznej |
| type: | journal article |
| subtype: | academic paper |
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Projekt „Repozytorium otwartego dostępu do dorobku naukowego i dydaktycznego UJ” współfinansowany w ramach poddziałania 2.3.1 „Cyfrowe udostępnianie zasobów nauki” Programu Operacyjnego Polska Cyfrowa z Europejskiego Funduszu Rozwoju Regionalnego i budżetu państwa na podstawie umowy o dofinansowanie nr POPC.02.03.01-00-0030/17-00
