Repozytorium Uniwersytetu Jagiellońskiego
Tyrosinase biosensor for benzoic acid inhibition-based determination with the use of a flow-batch monosegmented sequential injection system
Tyrosinase biosensor for benzoic acid inhibition-based ...
Opis pozycji
| tytuł: | Tyrosinase biosensor for benzoic acid inhibition-based determination with the use of a flow-batch monosegmented sequential injection system |
| autor: | Kochana Jolanta  , Kozak Joanna , Skrobisz Agata, Woźniakiewicz Michał
|
| tytuł czasopisma: | Talanta |
| tytuł numeru: | Selected Papers presented at the 17th ICFIA, Krakow, Poland (2011) |
| tom: | 96 |
| data wydania: | 2012 |
| strony: | 147-152 |
| ISSN: |
0039-9140 |
| eISSN: |
1873-3573 |
| DOI: | |
| język: | angielski |
| język czasopisma: | angielski |
| abstrakt w j. angielskim: | Amperometric tyrosinase based biosensor for benzoic acid determination with the use of a flow-batch monosegmented sequential injection system is proposed. The functioning of the biosensor is based on the inhibition effect of benzoic acid on the biocatalytic activity of tyrosinase, polyphenol oxidase. In the biosensor, tyrosinase is entrapped in titania gel modified with multiwalled carbon nanotubes (MWCNT) and Nafion. The procedure of optimization of experimental conditions affecting the biosensor response, as well as its analytical characteristics and results of the approach verification using synthetic samples and a reference material are presented. The developed biosensor exhibits linear range up to 2.46 M, good sensitivity toward benzoic acid (1.06 A M − 1 ) and low detection limit (0.03 M). The approach has been applied to benzoic acid determination in a real beverage sample giving the result consistent with that obtained using the HPLC method. |
| słowa kluczowe w j. angielskim: | benzoic acid, inhibition, sequential injection technique, tyrosinase, amperometry |
| punktacja wydziałowa: | 40 |
| wydział:instytut/zakład/katedra: | Wydział Chemii : Zakład Chemii Analitycznej |
| typ: | artykuł w czasopiśmie |
| podtyp: | artykuł |
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