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Interaction of apo-transferrin with anticancer ruthenium complexes NAMI-A and its reduced form

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Interaction of apo-transferrin with anticancer ruthenium complexes NAMI-A and its reduced form

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dc.contributor.author Mazuryk, Olga [SAP14006163] pl
dc.contributor.author Kurpiewska, Katarzyna [SAP11019445] pl
dc.contributor.author Lewiński, Krzysztof [SAP11011257] pl
dc.contributor.author Stochel, Grażyna [SAP11011061] pl
dc.contributor.author Brindell, Małgorzata [SAP11117158] pl
dc.date.accessioned 2015-08-21T11:27:46Z
dc.date.available 2015-08-21T11:27:46Z
dc.date.issued 2012 pl
dc.identifier.issn 0162-0134 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/14825
dc.language eng pl
dc.title Interaction of apo-transferrin with anticancer ruthenium complexes NAMI-A and its reduced form pl
dc.type JournalArticle pl
dc.description.physical 11-18 pl
dc.description.additional Bibliogr. s. 18 pl
dc.abstract.en NAMI-Ai.e.(ImH)[ trans -RuCl 4 (DMSO)(Im)](whereImisimidazole)isaruthenium(III)complexwithpromising antimetastatic activity, which has been classi fi ed for II phase clinical trial. In this study, its binding properties to- wardapo-transferrin(apo-Tf)withregardtoitshydrolyticandredoxbehavioraresystematicallyinvestigatedby the use of fl uorescence spectroscopy. The reaction of NAMI-A and its reduced form with apo-Tf is proceeded by formation of aqua derivativesand thepresenceofat least onelabileaqua ligand issuf fi cient to form adducts.It is found that presence of bicarbonate is not necessary for interaction of studied ruthenium complexes with apo-Tf. The calculated association constants for both NAMI-A and its reduced form are very similar with the values of 1.28×10 4 M − 1 and 1.36×10 4 M − 1 at 37 °C, respectively however, the reduced derivatives reach the equilib- rium ca. 8 – 10 times slower. The percentage of ruthenium content in protein fractions separated from protein-unbounded ruthenium by using FPLC (fast protein liquid chromatography) method is rather high and depends on redox state of the complex, for most samples is found higher for reduced species pl
dc.subject.en NAMI-A pl
dc.subject.en apo-transferrin pl
dc.subject.en association constant pl
dc.subject.en fluorescence quenching pl
dc.subject.en separation on FPLC pl
dc.subject.en ruthenium content pl
dc.description.volume 116 pl
dc.description.points 35 pl
dc.identifier.doi 10.1016/j.jinorgbio.2012.07.017 pl
dc.identifier.eissn 1873-3344 pl
dc.title.journal Journal of Inorganic Biochemistry pl
dc.language.container eng pl
dc.affiliation Wydział Chemii : Zakład Chemii Nieorganicznej pl
dc.affiliation Wydział Chemii : Zakład Krystalochemii i Krystalofizyki pl
dc.subtype Article pl
dc.rights.original bez licencji pl
.pointsMNiSW [2012 A]: 35


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