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Pseudomonas aeruginosa biofilm is a potent inducer of phagocyte hyperinflammation

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Pseudomonas aeruginosa biofilm is a potent inducer of phagocyte hyperinflammation

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dc.contributor.author Ciszek-Lenda, Marta [SAP20008509] pl
dc.contributor.author Strus, Magdalena [SAP20008032] pl
dc.contributor.author Walczewska, Maria [SAP20008458] pl
dc.contributor.author Majka, Grzegorz [SAP20017844] pl
dc.contributor.author Machul-Żwirbla, Agnieszka pl
dc.contributor.author Mikołajczyk, Diana pl
dc.contributor.author Górska-Frączek, Sabina pl
dc.contributor.author Gamian, Andrzej pl
dc.contributor.author Chain, Benjamin M. pl
dc.contributor.author Marcinkiewicz, Janusz [SAP20000603] pl
dc.date.accessioned 2020-01-17T10:07:58Z
dc.date.available 2020-01-17T10:07:58Z
dc.date.issued 2019 pl
dc.identifier.issn 1023-3830 pl
dc.identifier.uri https://ruj.uj.edu.pl/xmlui/handle/item/145991
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Pseudomonas aeruginosa biofilm is a potent inducer of phagocyte hyperinflammation pl
dc.type JournalArticle pl
dc.description.physical 397-413 pl
dc.abstract.en Objective Pseudomonas aeruginosa effectively facilitate resistance to phagocyte killing by biofilm formation. However,b the cross talk between biofilm components and phagocytes is still unclear. We hypothesize that a biofilm provides a concentrated extracellular source of LPS, DNA and exopolysaccharides (EPS), which polarize neighbouring phagocytes into an adverse hyperinflammatory state of activation. Methods We measured the release of a panel of mediators produced in vitro by murine neutrophils and macrophages exposed to various biofilm components of P. aeruginosa cultures. Results We found that conditioned media from a high biofilm-producing strain of P. aeruginosa, PAR5, accumulated high concentrations of extracellular bacterial LPS, DNA and EPS by 72 h. These conditioned media induced phagocytes to release a hyperinflammatory pattern of mediators, with enhanced levels of $TNF-\alpha$, IL-6, IL12p40, $PGE_{2}$ and NO. Moreover, the phagocytes also upregulated COX-2 and iNOS with no influence on the expression of arginase-1. Conclusions Phagocytes exposed to biofilm microenvironment, called by us biofilm-associated neutrophils/macrophages (BANs/BAMs), display secretory properties similar to that of N1/M1-type phagocytes. These results suggest that in vivo high concentrations of LPS and DNA, trapped in biofilm by EPS, might convert infiltrating phagocytes into cells responsible for tissue injury without direct contact with bacteria and phagocytosis. pl
dc.subject.en biofilm pl
dc.subject.en hyperinflammation pl
dc.subject.en neutrophils pl
dc.subject.en P. aeruginosa pl
dc.subject.en LPS pl
dc.subject.en DNA pl
dc.description.volume 68 pl
dc.description.number 5 pl
dc.description.points 70 pl
dc.identifier.doi 10.1007/s00011-019-01227-x pl
dc.identifier.eissn 1420-908X pl
dc.title.journal Inflammation Research pl
dc.language.container eng pl
dc.affiliation Wydział Lekarski : Zakład Immunologii pl
dc.affiliation Wydział Lekarski : Zakład Bakteriologii,Ekologii Drobnoustrojów i Parazytologii pl
dc.affiliation Wydział Lekarski : Katedra Immunologii pl
dc.subtype Article pl
dc.rights.original CC-BY; inne; ostateczna wersja wydawcy; w momencie opublikowania; 0 pl
dc.identifier.project ROD UJ / OP pl
dc.cm.id 93985
dc.cm.date 2020-01-07
.pointsMNiSW [2019 A]: 70


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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa