title:
|
Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy |
author: |
Rubiś Paweł , Wiśniowska-Śmiałek Sylwia , Wypasek Ewa , Biernacka-Fijałkowska Barbara, Rudnicka-Sosin Lucyna, Dziewięcka Ewa, Matusik Patrycja S., Khachatryana Lusine, Karabinowska Aleksandra, Kozanecki Artur, Tomkiewicz-Pająk Lidia , Podolec Piotr
|
journal title:
|
Inflammation Research |
volume: |
65 |
issue:
|
12 |
date of publication
:
|
2016 |
pages:
|
941-949 |
ISSN: |
1023-3830
|
eISSN: |
1420-908X
|
DOI: |
10.1007/s00011-016-0977-3
|
language: |
English |
journal language:
|
English |
abstract in English: |
Background Fibrosis of extracellular matrix (ECM) in
dilated cardiomyopathy (DCM) corresponds to the
myocardial over-production of various types of collagens.
However, mechanism of this process is poorly understood.
Objective To investigate whether enhanced metabolism of
ECM occur in DCM.
Methods Seventy consecutive DCM patients (pts)
(48 ± 12.1 years, EF 24.4 ± 7.4 %) and 20 healthy volunteers
were studied. Based on symptoms duration, pts
were divided into new-onset (n = 35, 6 months) and
chronic DCM (n = 35, >6 months). Markers of collagen
type I and III synthesis-procollagen type I carboxy- and
amino-terminal peptides (PICP and PINP) and procollagen
type III carboxy- and amino-terminal peptides (PIIICP and
PIIINP), collagen 1 (col-1), ECM metabolism controlling
factors-tumor growth factor beta-1 (TGF1-b), connective
tissue growth factor (CTGF), and ECM degradation
enzymes-matrix metalloproteinases (MMP-2, MMP-9)
and their tissue inhibitor (TIMP-1) were measured in serum. All pts underwent right ventricular endomyocardial
biopsy to study ECM fibrosis.
Results The presence of fibrosis was detected in 24
(34.3 %) pts and was more prevalent in chronic DCM
[17 (48.6 %) vs. 7 (20 %), p\0.01]. The levels of
PIIINP [4.41 (2.17-6.08) vs. 3.32 (1.69-5.02) ng/ml,
p\0.001], CTGF [3.82 (0.48-23.87) vs. 2.37
(0.51-25.32) ng/ml, p\0.01], MMP-2 [6.06 (2.72-14.8)
vs. 4.43 (2.27=7.4) ng/ml, p\0.001], MMP-9 [1.98
(0.28-9.25) vs. 1.01 (0.29-3.59) ng/ml, p\0.002)], and
TIMP-1 [15.29 (1.8-36.17) vs. 2.61 (1.65-24.09) ng/ml,
p\0.004] were significantly higher in DCM, whereas
levels of col-1 [57.7 (23.1-233.4) vs. 159.4 (31.2-512.9)
pg/ml, p\0.001] were significantly lower in DCM
compared to controls. There were no differences in all
measured serum markers of ECM metabolism between
newonset and chronic DCM and as well as fibrosis
positive and negative pts. Fibrosis was weakly correlated
only with the duration of DCM (r = 0.23, p\0.05),
however, not a single serum marker of fibrosis correlated
with fibrosis. Neither unadjusted nor adjusted models,
constructed from serum markers of ECM metabolism,
predicted the probability of myocardial fibrosis.
Conclusions Dynamics of ECM turnover in DCM is high,
which is reflected by the increased levels CTGF and
degradation enzymes. Synthesis of collagen type III prevailed
over collagen type I. ECM metabolism was not
different in DCM regardless of the duration of the disease
and status of myocardial fibrosis. Serum markers of ECM
metabolism were found not to be useful for the prediction
of myocardial fibrosis in DCM. |
keywords in English: |
dilated cardiomyopathy, extracellular matrix, fibrosis, markers, collagen, biopsy |
departmental parameterization: |
20 |
affiliation: |
Wydział Lekarski : Instytut Kardiologii |
type: |
journal article |
subtype: |
academic paper |
punktacja MNiSW [2016 A]: 20