Pretreatment with obestatin inhibits the development of acetic acid-induced colitis in rats
author:
Matuszyk Aleksandra , Ceranowicz Piotr , Warzecha Zygmunt , Cieszkowski Jakub , Gałązka Krystyna , Bonior Joanna , Jaworek Jolanta , Konturek Peter Christopher, Gil Krzysztof , Dembiński Artur
Introduction: Obestatin is a 23-amino acid peptide derived from proghrelin,
a common prohormone for ghrelin and obestatin. Previous studies have
shown that obestatin exhibits some protective and therapeutic effects in
the pancreas and stomach. The aim of this study was to examine the effect
of pretreatment with obestatin on the development of acetic acid-induced
colitis.
Material and methods: Studies were performed on Wistar rats. Before induction of colitis, rats were treated intraperitoneally with saline or obestatin, administered twice at a dose of 4, 8 or 16 nmol/kg/dose. The first dose
of saline or obestatin was administered 8 h before the induction of colitis,
the second one 7 h after the first dose. Colitis was induced by enema with
1 ml of 4% acetic acid solution. The severity of colitis was assessed 1 or 24 h
after administration of enema.
Results: Pretreatment with obestatin administered at a dose of 8 or 16 nmol/
kg/dose significantly reduced the area of mucosal damage evoked by enema
with acetic acid (p < 0.05). This effect was accompanied by an improvement
of mucosal blood flow and DNA synthesis in the colon. Moreover, obestatin
administered at a dose of 8 or 16 nmol/kg/dose significantly reduced mucosal concentration of IL-1β and activity of myeloperoxidase (p < 0.05).
Conclusions: Pretreatment with obestatin exhibited a protective effect in
the colon, leading to a reduction of colonic damage in acetic acid-induced
colitis. This effect was associated with an improvement of mucosal blood
flow, an increase in mucosal cell proliferation, and a decrease in local inflammation.
Wydział Lekarski : Zakład Anatomii, Wydział Lekarski : Zakład Fizjologii Klinicznej, Wydział Lekarski : Zakład Patomorfologii Klinicznej i Doświadczalnej, Wydział Lekarski : Zakład Patofizjologii, Wydział Nauk o Zdrowiu : Instytut Fizjoterapii
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