Repozytorium Uniwersytetu Jagiellońskiego

Differentiation between single bladder cancer cells using principal component analysis of time-of-flight secondary ion mass spectrometry

Differentiation between single bladder cancer cells ...

Metadane (Dublin Core)

dc.contributor.author Gostek, Justyna pl
dc.contributor.author Awsiuk, Kamil [SAP14001148] pl
dc.contributor.author Pabijan, Joanna pl
dc.contributor.author Rysz, Jakub [SAP11116295] pl
dc.contributor.author Budkowski, Andrzej [SAP11011875] pl
dc.contributor.author Lekka, Małgorzata pl
dc.date.accessioned 2015-07-13T14:47:22Z
dc.date.available 2015-07-13T14:47:22Z
dc.date.issued 2015 pl
dc.identifier.issn 0003-2700 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/12630
dc.language eng pl
dc.rights Dodaję tylko opis bibliograficzny *
dc.rights.uri *
dc.title Differentiation between single bladder cancer cells using principal component analysis of time-of-flight secondary ion mass spectrometry pl
dc.type JournalArticle pl
dc.description.physical 3195-3201 pl
dc.abstract.en Time-of-flight-secondary ion mass spectrometry (TOF-SIMS) mass spectra measurements combined with an appropriate sample preparation protocol are the powerful tools to obtain unique information about the chemical composition of biological materials. In our studies, two questions were addressed, i.e., whether it is possible to develop a fixative-based sample preparation protocol and whether it allows one to distinguish between cells originating from various stages of cancer progression. Therefore, four human bladder cancer cell lines (with distinct malignancy degree) have been investigated. A chemical fixation protocol has been used for TOF-SIMS measurements, and mass spectra were obtained using a Bi_{3}+ primary ion beam. The principal component analysis (PCA) has been applied to analyze the whole range of mass spectra (without preselection of any particular masses) using two approaches of data preprocessing, namely, mean centering and autoscaling. The PC3 versus PC2 plot has showed significant differences between nonmalignant cancer cells and the cancerous ones for both of preprocessing approaches. The analysis of mass spectra of human bladder cells allows one to find a list of mass peaks with intensities significantly larger in cancerous bladder cells compared to nonmalignant cell cancer of the ureter (HCV29 cells). These findings show that TOF-SIMS in combination with PCA can be used to identify reference, human bladder cells from cancerous ones. pl
dc.description.volume 87 pl
dc.description.number 6 pl
dc.description.publication 0,5 pl
dc.identifier.doi 10.1021/ac504684n pl
dc.identifier.eissn 1520-6882 pl
dc.title.journal Analytical Chemistry pl
dc.language.container eng pl
dc.affiliation Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego pl
dc.subtype Article pl
dc.rights.original bez licencji pl
.pointsMNiSW [2015 A]: 45


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