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MHC influences infection with parasites and winter survival in the root vole Microtus oeconomus

MHC influences infection with parasites and winter ...

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dc.contributor.author Kloch, Agnieszka [SAP13037863] pl
dc.contributor.author Baran, Kamil pl
dc.contributor.author Buczek, Mateusz [SAP13037855] pl
dc.contributor.author Konarzewski, Marek pl
dc.contributor.author Radwan, Jacek [SAP11014051] pl
dc.date.accessioned 2015-07-02T11:52:42Z
dc.date.available 2015-07-02T11:52:42Z
dc.date.issued 2013 pl
dc.identifier.issn 0269-7653 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/11252
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title MHC influences infection with parasites and winter survival in the root vole Microtus oeconomus pl
dc.type JournalArticle pl
dc.description.physical 635-653 pl
dc.abstract.en Selective pressure from parasites is thought to maintain the polymorphism of major histocompatibility complex (MHC) genes. Although a number of studies have shown a relationship between the MHC and parasitic infections, the fitness consequences of such associations are less well documented. In the present paper, we characterised the variation in exon 2 of MHC class II DRB gene in the root vole and examined the effects of that gene on parasite prevalence and winter survival. We identified 18 unique exon 2 sequences, which translated into 10 unique amino acid sequences. Phylogenetic analysis revealed the presence of three distinct clusters, and allele distributions among these individuals suggested that the clusters correspond to three different loci. Although the rate of synonymous substitutions (dS) exceeded the rate of nonsynonymous substitutions (dN) across sequences, implying purifying selection, dN was significantly elevated at antigen-binding sites, suggesting that these sites could be under positive selection. Screening for parasites revealed a moderate prevalence of infection with gastrointestinal parasites (24 % infected), but a high infection rate for blood parasites (56 % infected). Infection with the blood parasite Babesia ssp. decreased survival almost twofold (25.7 vs. 13.9 %). Animals possessing the amino acid sequence AA*08 survived better than others (44.9 vs. 22 %), and they were infected with Babesia ssp. less often (13.9 vs 25.7 %). In contrast, individuals carrying allele AA*05 were infected more often (31.7 vs. 15.3 %). Heterozygosity at one of the putative loci was associated with a lower probability of infection with Babesia ssp., but at the other locus, the association was reversed. The unexpected latter result could be at least partly explained by the increased frequency of the susceptible allele AA*05 among heterozygotes. Overall, we demonstrate that infection with Babesia ssp. is a strong predictor of winter survival and that MHC genes are important predictors of infection status as well as survival in the root vole. pl
dc.subject.en MHC pl
dc.subject.en Microtus oeconomus pl
dc.subject.en survival pl
dc.subject.en infection pl
dc.subject.en parasites pl
dc.subject.en Babesia pl
dc.subject.en next-generation sequencing pl
dc.description.volume 27 pl
dc.description.number 3 pl
dc.identifier.doi 10.1007/s10682-012-9611-1 pl
dc.identifier.eissn 1573-8477 pl
dc.title.journal Evolutionary Ecology pl
dc.language.container eng pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Nauk o Środowisku pl
dc.subtype Article pl
dc.rights.original CC-BY; inne; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
dc.pbn.affiliation USOS38535:UJ.WBl; USOS38521:UJ.WBl; pl
.pointsMNiSW [2013 A]: 25

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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa