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Multi-methodological insight into the vessel wall cross-section : Raman and AFM imaging combined with immunohistochemical staining

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Multi-methodological insight into the vessel wall cross-section : Raman and AFM imaging combined with immunohistochemical staining

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dc.contributor.author Pilarczyk, Marta pl
dc.contributor.author Ryguła, Anna [SAP14002897] pl
dc.contributor.author Mateuszuk, Łukasz [SAP14003983] pl
dc.contributor.author Chłopicki, Stefan [SAP20000969] pl
dc.contributor.author Barańska, Małgorzata [SAP11016906] pl
dc.contributor.author Kaczor, Agnieszka [SAP11018145] pl
dc.date.accessioned 2015-06-26T15:07:23Z
dc.date.available 2015-06-26T15:07:23Z
dc.date.issued 2013 pl
dc.identifier.issn 2212-8794 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/10545
dc.language eng pl
dc.title Multi-methodological insight into the vessel wall cross-section : Raman and AFM imaging combined with immunohistochemical staining pl
dc.type JournalArticle pl
dc.description.physical 191-197 pl
dc.abstract.en The vascular wall cross-section was submitted to multiparametric analysis with the application of several micro-scale methods: Raman and atomic force microscopy (AFM) imaging as well as immunohistochemical (IHC) staining. Both IHC staining as a standard reference method and Raman imaging allowed for visualization of tissue components such as elastin fibers or cell nuclei, moreover, Raman spectroscopy provided specific biochemical information about sample composition. Application of AFM complemented the chemical information with the data about the topography of the studied sample. Developing such a methodology allows for the multiparameter description of the vessel wall status that could be useful to monitor ex vivo the disease progression and effects of treatment in experimental diabetes, atherosclerosis or hypertension. pl
dc.description.volume 2 pl
dc.description.number 3 pl
dc.description.publication 0,79 pl
dc.identifier.doi 10.3233/BSI-130048 pl
dc.identifier.eissn 2212-8808 pl
dc.title.journal Biomedical Spectroscopy and Imaging pl
dc.language.container eng pl
dc.affiliation Wydział Lekarski : Zakład Farmakologii Doświadczalnej pl
dc.affiliation Wydział Chemii : Zakład Fizyki Chemicznej pl
dc.subtype Article pl
dc.rights.original bez licencji pl


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