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HGF/SF increases number of skin melanocytes but does not alter quality or quantity of follicular melanogenesis

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HGF/SF increases number of skin melanocytes but does not alter quality or quantity of follicular melanogenesis

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dc.contributor.author Wolnicka-Głubisz, Agnieszka [SAP11017905] pl
dc.contributor.author Pecio, Anna [SAP11010457] pl
dc.contributor.author Podkowa, Dagmara [SAP12018247] pl
dc.contributor.author Płonka, Przemysław [SAP11015581] pl
dc.contributor.author Grabacka, Maja pl
dc.date.accessioned 2015-06-25T09:50:13Z
dc.date.available 2015-06-25T09:50:13Z
dc.date.issued 2013 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/10322
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa *
dc.rights.uri https://creativecommons.org/licenses *
dc.title HGF/SF increases number of skin melanocytes but does not alter quality or quantity of follicular melanogenesis pl
dc.type JournalArticle pl
dc.abstract.en Melanins are an important factor determining the vulnerability of mammalian skin to UV radiation and thus to UV-induced skin cancers. Transgenic mice overexpressing hepatocyte growth factor/scatter factor (HGF/SF) have extra-follicular dermal melanocytes, notably in the papillary upper dermis, and are susceptible to UV-induced melanoma. Pigmented HGF/SF neonatal mice are more susceptible than albino HGF/SF animals to UVA -induced melanoma, indicating an involvement of melanin in melanoma formation. This raises the question of the effect of transgenic HGF/SF on melanization. We developed a methodology to accurately quantitate both the production of melanin and the efficiency of melanogenesis in normal, and HGF/SF transgenic mice in vivo. Skin and hair shafts of 5 day old and adult (3 week old) C57BL/6-HGF/SF and corresponding C57BL/6 wild type mice were investigated by electron paramagnetic resonance spectroscopy (EPR) to quantitate melanin, by transmission electron microscopy (TEM) for the presence of melanosomes, and by standard histology and by Western blotting and zymography to determine the expression and activity of melanogenesis-related proteins. Eumelanin but no phaeomelanin was detected in transgenic C57BL/6-HGF and C57BL/6 wild type mice. Transgenic HGF/SF overexpression did not change the type of melanin produced in the skin or hair, did not affect the terminal content of melanin production in standard samples of hair and did not influence hair cycle/morphogenesis-related changes in skin thickness. No melanocytes were found in the epidermis and no melanosomes were found in epidermal keratinocytes. HGF/SF transgenic mice thus lack the epidermal melanin UV-protection found in constitutively dark human skin. We conclude that melanocytes in the HGF/SF transgenic mouse, particularly in the papillary dermis, are vulnerable to UVA which interacts with eumelanin but not phaeomelanin to induce melanoma. pl
dc.description.volume 8 pl
dc.description.number 11 pl
dc.identifier.doi 10.1371/journal.pone.0074883 pl
dc.identifier.eissn 1932-6203 pl
dc.title.journal PLoS ONE pl
dc.language.container eng pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizyki pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Zoologii pl
dc.subtype Article pl
dc.identifier.articleid e74883 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2013 A]: 40


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Udzielam licencji. Uznanie autorstwa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa