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SERS-based monitoring of the intracellular pH in endothelial cells : the influence of the extracellular environment and tumour necrosis factor-
4-mercaptobenzoate
benzoic acid derivative
gold
metal nanoparticle
thiol derivative
tumor necrosis factor alpha
chemistry
cytology
drug effects
extracellular space
human
intracellular space
molecular imaging
pH
Raman spectrometry
umbilical vein endothelial cell
Benzoates
Extracellular Space
Gold
Human Umbilical Vein Endothelial Cells
Humans
Hydrogen-Ion Concentration
Intracellular Space
Metal Nanoparticles
Molecular Imaging
Spectrum Analysis
Raman
Sulfhydryl Compounds
Tumor Necrosis Factor-alpha
The intracellular pH plays an important role in various cellular processes. In this work, we describe a method for monitoring of the intracellular pH in endothelial cells by using surface enhanced Raman spectroscopy (SERS) and 4-mercaptobenzoic acid (MBA) anchored to gold nanoparticles as pH-sensitive probes. Using the Raman microimaging technique, we analysed changes in intracellular pH induced by buffers with acid or alkaline pH, as well as in endothelial inflammation induced by tumour necrosis factor-α (TNFα). The targeted nanosensor enabled spatial pH measurements revealing distinct changes of the intracellular pH in endosomal compartments of the endothelium. Altogether, SERS-based analysis of intracellular pH proves to be a promising technique for a better understanding of intracellular pH regulation in various subcellular compartments.
| dc.abstract.en | The intracellular pH plays an important role in various cellular processes. In this work, we describe a method for monitoring of the intracellular pH in endothelial cells by using surface enhanced Raman spectroscopy (SERS) and 4-mercaptobenzoic acid (MBA) anchored to gold nanoparticles as pH-sensitive probes. Using the Raman microimaging technique, we analysed changes in intracellular pH induced by buffers with acid or alkaline pH, as well as in endothelial inflammation induced by tumour necrosis factor-α (TNFα). The targeted nanosensor enabled spatial pH measurements revealing distinct changes of the intracellular pH in endosomal compartments of the endothelium. Altogether, SERS-based analysis of intracellular pH proves to be a promising technique for a better understanding of intracellular pH regulation in various subcellular compartments. | pl |
| dc.affiliation | Wydział Chemii : Zakład Fizyki Chemicznej | pl |
| dc.affiliation | Wydział Lekarski : Zakład Farmakologii Doświadczalnej | pl |
| dc.affiliation | Pion Rektora : Jagiellońskie Centrum Rozwoju Leków | pl |
| dc.cm.id | 70184 | |
| dc.contributor.author | Jaworska, Aleksandra - 107004 | pl |
| dc.contributor.author | Jamieson, Lauren E. | pl |
| dc.contributor.author | Małek, Kamilla - 130284 | pl |
| dc.contributor.author | Campbell, Colin J. | pl |
| dc.contributor.author | Choo, Jaebum | pl |
| dc.contributor.author | Chłopicki, Stefan - 128995 | pl |
| dc.contributor.author | Barańska, Małgorzata - 127224 | pl |
| dc.date.accessioned | 2015-06-24T07:50:02Z | |
| dc.date.available | 2015-06-24T07:50:02Z | |
| dc.date.issued | 2015 | pl |
| dc.description.number | 7 | pl |
| dc.description.physical | 2321-2329 | pl |
| dc.description.points | 40 | pl |
| dc.description.volume | 140 | pl |
| dc.identifier.doi | 10.1039/c4an01988a | pl |
| dc.identifier.eissn | 1364-5528 | pl |
| dc.identifier.issn | 0003-2654 | pl |
| dc.identifier.uri | http://ruj.uj.edu.pl/xmlui/handle/item/10172 | |
| dc.language | eng | pl |
| dc.language.container | eng | pl |
| dc.rights.licence | bez licencji | |
| dc.subject.en | 4-mercaptobenzoate | pl |
| dc.subject.en | benzoic acid derivative | pl |
| dc.subject.en | gold | pl |
| dc.subject.en | metal nanoparticle | pl |
| dc.subject.en | thiol derivative | pl |
| dc.subject.en | tumor necrosis factor alpha | pl |
| dc.subject.en | chemistry | pl |
| dc.subject.en | cytology | pl |
| dc.subject.en | drug effects | pl |
| dc.subject.en | extracellular space | pl |
| dc.subject.en | human | pl |
| dc.subject.en | intracellular space | pl |
| dc.subject.en | molecular imaging | pl |
| dc.subject.en | pH | pl |
| dc.subject.en | Raman spectrometry | pl |
| dc.subject.en | umbilical vein endothelial cell | pl |
| dc.subject.en | Benzoates | pl |
| dc.subject.en | Extracellular Space | pl |
| dc.subject.en | Gold | pl |
| dc.subject.en | Human Umbilical Vein Endothelial Cells | pl |
| dc.subject.en | Humans | pl |
| dc.subject.en | Hydrogen-Ion Concentration | pl |
| dc.subject.en | Intracellular Space | pl |
| dc.subject.en | Metal Nanoparticles | pl |
| dc.subject.en | Molecular Imaging | pl |
| dc.subject.en | Spectrum Analysis | pl |
| dc.subject.en | Raman | pl |
| dc.subject.en | Sulfhydryl Compounds | pl |
| dc.subject.en | Tumor Necrosis Factor-alpha | pl |
| dc.subtype | Article | pl |
| dc.title | SERS-based monitoring of the intracellular pH in endothelial cells : the influence of the extracellular environment and tumour necrosis factor-$\alpha$ | pl |
| dc.title.journal | Analyst | pl |
| dc.type | JournalArticle | pl |
| dspace.entity.type | Publication |
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