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Interactions of alkylphosphocholines with model membranes : the Langmuir monolayer study


Interactions of alkylphosphocholines with model membranes : the Langmuir monolayer study

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dc.contributor.author Wnętrzak, Anita [SAP14013174] pl
dc.contributor.author Łątka, Kazimierz [SAP11007627] pl
dc.contributor.author Dynarowicz-Łątka, Patrycja [SAP11012997] pl
dc.date.accessioned 2015-06-23T09:53:39Z
dc.date.available 2015-06-23T09:53:39Z
dc.date.issued 2013 pl
dc.identifier.issn 0022-2631 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/10085
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Interactions of alkylphosphocholines with model membranes : the Langmuir monolayer study pl
dc.type JournalArticle pl
dc.description.physical 453-466 pl
dc.identifier.weblink https://link.springer.com/content/pdf/10.1007%2Fs00232-013-9557-4.pdf pl
dc.abstract.en Alkylphosphocholines (APCs) belong to a class of synthetic antitumor lipids, which are new-generation anticancer agents. In contrast to traditional antitumor drugs, they do not attack the cell nucleus but, rather, the cellular membrane; however, their mechanism of action is not fully understood. This work compared the interactions of selected APCs [namely, hexadecylphosphocholine (miltefosine), octadecylphosphocholine and erucylphosphocholine] with the most important membrane lipids [cholesterol, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)] and examined their influence on a model membrane of tumor and normal cells. As a simple model of membranes, Langmuir monolayers prepared by mixing cholesterol either with a saturated phosphatidylcholine (DPPC), for a normal cell membrane, or with an unsaturated one (POPC), for a tumor cell membrane, have been applied. The APC–lipid interactions, based on experimental surface pressure (π) versus mean molecular area (A) isotherms, were analyzed qualitatively (with mean molecular area values) as well as quantitatively (with the \Delta G^{exc} function). Strong attractive interactions were observed for mixtures of APCs with cholesterol, contrary to the investigated phosphatidylcholines, for which the interactions were found to be weak with a tendency to separation of film components. In ternary monolayers it has been found that the investigated model systems (cholesterol/DPPC/APC vs cholesterol/POPC/APC) differ significantly as regards the interactions between film-forming molecules. The results demonstrate stronger interactions between the components of cholesterol/POPC/APC monolayers compared to cholesterol/POPC film, mimicking tumor cell membranes. In contrast, the interactions in cholesterol/DPPC/APC films were found to be weaker than those in the cholesterol/DPPC system, serving as a model of healthy cell membranes, thus proving that the incorporation of APCs is, from a thermodynamic point of view, unfavorable for binary cholesterol/DPPC monolayers. It can be concluded that the composition of healthy cell membranes is a natural barrier preventing the incorporation of APCs into normal cells. pl
dc.subject.en alkylphosphocholine pl
dc.subject.en model membrane pl
dc.subject.en Langmuir monolayer pl
dc.subject.en interaction pl
dc.description.volume 246 pl
dc.description.number 6 pl
dc.description.publication 1 pl
dc.identifier.doi 10.1007/s00232-013-9557-4 pl
dc.identifier.eissn 1432-1424 pl
dc.title.journal The Journal of Membrane Biology pl
dc.language.container eng pl
dc.date.accession 2019-02-04 pl
dc.affiliation Wydział Chemii : Zakład Chemii Ogólnej pl
dc.affiliation Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego pl
dc.subtype Article pl
dc.rights.original CC-BY; inne; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2013 A]: 20

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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa