Spin trapping of nitric oxide by hemoglobin and ferrous diethyldithiocarbamate in model tumors differing in vascularization

2024
journal article
article
dc.abstract.enAnimal tumors serve as reasonable models for human cancers. Both human and animal tumors often reveal triplet EPR signals of nitrosylhemoglobin (HbNO) as an effect of nitric oxide formation in tumor tissue, where NO is complexed by Hb. In search of factors determining the appearance of nitrosylhemoglobin (HbNO) in solid tumors, we compared the intensities of electron paramagnetic resonance (EPR) signals of various iron–nitrosyl complexes detectable in tumor tissues, in the presence and absence of excess exogenous iron(II) and diethyldithiocarbamate (DETC). Three types of murine tumors, namely, L5178Y lymphoma, amelanotic Cloudman S91 melanoma, and Ehrlich carcinoma (EC) growing in DBA/2 or Swiss mice, were used. The results were analyzed in the context of vascularization determined histochemically using antibodies to CD31. Strong HbNO EPR signals were found in melanoma, i.e., in the tumor with a vast amount of a hemorrhagic necrosis core. Strong $Fe(DETC)_{2}NO$ signals could be induced in poorly vascularized EC. In L5178Y, there was a correlation between both types of signals, and in addition, $Fe(RS)_{2}(NO)_{2}$ signals of non-heme iron–nitrosyl complexes could be detected. We postulate that HbNO EPR signals appear during active destruction of well-vascularized tumor tissue due to hemorrhagic necrosis. The presence of iron–nitrosyl complexes in tumor tissue is biologically meaningful and defines the evolution of complicated tumor–host interactions.
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizyki i Biologii Nowotworów
dc.contributor.authorSzczygieł, Dariusz - 255556
dc.contributor.authorSzczygieł, Małgorzata - 200073
dc.contributor.authorŁaś, Anna
dc.contributor.authorElas, Martyna - SAP11015756
dc.contributor.authorZuziak, Roxana
dc.contributor.authorPłonka, Beata - SAP11016806
dc.contributor.authorPłonka, Przemysław - 131459
dc.date.accessioned2024-04-22T12:40:35Z
dc.date.available2024-04-22T12:40:35Z
dc.date.issued2024
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalBeata Płonka podpisana: Beata K. Płonka. Przemysław Płonka podpisany: Przemysław K. Płonka. Bibliogr.
dc.description.number8
dc.description.versionostateczna wersja wydawcy
dc.description.volume25
dc.identifier.articleid4172
dc.identifier.doi10.3390/ijms25084172
dc.identifier.issn1422-0067
dc.identifier.urihttps://ruj.uj.edu.pl/handle/item/330675
dc.languageeng
dc.language.containereng
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.typeotwarte czasopismo
dc.subject.enEPR
dc.subject.enESR
dc.subject.enhemoglobin
dc.subject.enDETC
dc.subject.enNO
dc.subject.enhemorrhagic necrosis
dc.subject.enmelanoma
dc.subject.enlymphoma
dc.subject.enEhrlich carcinoma
dc.subtypeArticle
dc.titleSpin trapping of nitric oxide by hemoglobin and ferrous diethyldithiocarbamate in model tumors differing in vascularization
dc.title.journalInternational Journal of Molecular Sciences
dc.typeJournalArticle
dspace.entity.typePublicationen
dc.abstract.en
Animal tumors serve as reasonable models for human cancers. Both human and animal tumors often reveal triplet EPR signals of nitrosylhemoglobin (HbNO) as an effect of nitric oxide formation in tumor tissue, where NO is complexed by Hb. In search of factors determining the appearance of nitrosylhemoglobin (HbNO) in solid tumors, we compared the intensities of electron paramagnetic resonance (EPR) signals of various iron–nitrosyl complexes detectable in tumor tissues, in the presence and absence of excess exogenous iron(II) and diethyldithiocarbamate (DETC). Three types of murine tumors, namely, L5178Y lymphoma, amelanotic Cloudman S91 melanoma, and Ehrlich carcinoma (EC) growing in DBA/2 or Swiss mice, were used. The results were analyzed in the context of vascularization determined histochemically using antibodies to CD31. Strong HbNO EPR signals were found in melanoma, i.e., in the tumor with a vast amount of a hemorrhagic necrosis core. Strong $Fe(DETC)_{2}NO$ signals could be induced in poorly vascularized EC. In L5178Y, there was a correlation between both types of signals, and in addition, $Fe(RS)_{2}(NO)_{2}$ signals of non-heme iron–nitrosyl complexes could be detected. We postulate that HbNO EPR signals appear during active destruction of well-vascularized tumor tissue due to hemorrhagic necrosis. The presence of iron–nitrosyl complexes in tumor tissue is biologically meaningful and defines the evolution of complicated tumor–host interactions.
dc.affiliation
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizyki i Biologii Nowotworów
dc.contributor.author
Szczygieł, Dariusz - 255556
dc.contributor.author
Szczygieł, Małgorzata - 200073
dc.contributor.author
Łaś, Anna
dc.contributor.author
Elas, Martyna - SAP11015756
dc.contributor.author
Zuziak, Roxana
dc.contributor.author
Płonka, Beata - SAP11016806
dc.contributor.author
Płonka, Przemysław - 131459
dc.date.accessioned
2024-04-22T12:40:35Z
dc.date.available
2024-04-22T12:40:35Z
dc.date.issued
2024
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additional
Beata Płonka podpisana: Beata K. Płonka. Przemysław Płonka podpisany: Przemysław K. Płonka. Bibliogr.
dc.description.number
8
dc.description.version
ostateczna wersja wydawcy
dc.description.volume
25
dc.identifier.articleid
4172
dc.identifier.doi
10.3390/ijms25084172
dc.identifier.issn
1422-0067
dc.identifier.uri
https://ruj.uj.edu.pl/handle/item/330675
dc.language
eng
dc.language.container
eng
dc.rights
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.en
EPR
dc.subject.en
ESR
dc.subject.en
hemoglobin
dc.subject.en
DETC
dc.subject.en
NO
dc.subject.en
hemorrhagic necrosis
dc.subject.en
melanoma
dc.subject.en
lymphoma
dc.subject.en
Ehrlich carcinoma
dc.subtype
Article
dc.title
Spin trapping of nitric oxide by hemoglobin and ferrous diethyldithiocarbamate in model tumors differing in vascularization
dc.title.journal
International Journal of Molecular Sciences
dc.type
JournalArticle
dspace.entity.typeen
Publication

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